Torque teno virus: Its prevalence and isotypes in North India

Table 1 Prevalence of TTV-DNA in different liver & renal disease groups

Disease group	Number of samples	TTV-DNA positivity n (%)
Acute viral hepatitis (AVH)	126	29 (23.0)
Chronic viral hepatitis (CVH)	111	26 (23.4)
Cirrhosis (CIR)	132	46 (34.8)
Fulminant hepatic failure (FHF)	51	15 (29.4)
Hepatocellular carcinoma (HCC)	93	21 (22.6)
Chronic renal failure (CRF)	65	38 (58.5)
Healthy controls	100	27 (27.0)

Table 2 Relation between TTV infection & ALT level in different liver disease groups n (%)

Diseases group	TTV-DNA+ samples	ALT level (IU/L)
		50-200	201-400	401-600	> 600
AVH	29	17 (59)	8 (28)	0	4 (14)
CVH	26	18 (69)	6 (23)	0	2 (8)
CIR	46	35 (76)	19 (41)	0	2 (4)
FHF	15	2 (13)	6 (40)	1 (7)	6 (40)
HCC	21	8 (38)	5 (24)	3 (14)	5 (24)

Percent value was computed in comparison to total number positive for TTV-DNA.

Table 3 TTV genotype analysis by RFLP n (%)

Restrictionenzymes used	Number of cases treated	Digestion noticed	Possible TTV genotypes	Genotypes prevalence
Nde I	55	53	G1	53 (96.4)
Pst I	55	2	G2	2 (3.6)
Mse I	55	0	-	0
Nla III	55	0	-	0
Each sample was treated with four restriction enzymes (Nde I, Pst I, Mse I, Nla III) to determine genotyping of TTV following criteria mentioned in material and methods
The DNA remaining undigested with REs indicated absence of corresponding genotypes
There was no effect of Mse I and Nla III on TTV-DNA on digestion with these enzymes