Characterization of hepatic progenitors from human fetal liver during second trimester

Table 1 List of primer sequences used in the study

Genes	Primer sequence	Product size (bp)
CK18	Sense-TGGTACTCTCCTCAATCTGCTG	148
	Anti-sense-CTCTGGATTGACTGTGGAAGT
CK19	Sense-CCTGCGGGACAAGATTCTTG	326
	Anti-sense-ACGGGCGTTGTCGATCTG
Albumin	Sense-GCTTTGCCGAGGAGGGTAA	161
	Anti-sense-GTAGGCTGAGATGCTTTATGT
AFP	Sense-GCAAAGCTGAAAATGCAGTTGA	216
	Anti-sense-GGAAAGTTCGGGTCCCAAAA

Table 2 Expression of hepatic and progenitor markers on EpCAM +ve cells

Antibody	Group 1 (n = 5), cells%	Group 2 (n = 5), cells%	P
CD29	3.9 ± 1.3	1.9 ± 0.7	0.005
CD49f	2.1 ± 0.6	1.5 ± 0.7	0.372
CD90	3.5 ± 1.9	1.3 ± 0.3	0.044
Albumin	19.2 ± 3.1	21.3 ± 4.2	0.817
AFP	7.3 ± 0.6	7.2 ± 1.0	0.865
HLA-A, B, C (HLA Class I)	6.5 ± 2.4	8.2 ± 2.1	0.279
HLA-DR (HLA class II )	0.6 ± 0.4	0.35 ± 0.1	0.612