Biological impact of hepatitis B virus X-hepatitis C virus core fusion gene on human hepatocytes

Table 1 Sequences of gene primers

Target gene	Primer sequences	Product (bp)
HBV X gene	P1: 5’-ATCTGTCGACATGGCTGCTAGGCTGTGCT G-3’	465
	P2: 5’-CGCGGATATCTTAGGCAGAGGTGAAAAAGT TG-3’
HCV C gene	P3: 5’-ACTGGTCGACATGAGCACGAATCCTAAACCT C-3’	576
	P4: 5’-ACTGGATATCTTAGGCTGAAGCGGGCAC AG-3’
HBV X-Linker fragment	P1: 5’-ATCTGTCGACATGGCTGCTAGGCTGTGCT G-3’	510
	P2’: 5’-GCTGCCGCCACCGCCGCTTCCGCCACCGCCGCTTGCCACCGGCAGAGGTGAAAAAGTTGCA-3’
Linker-HCV C fragment	P3’: 5’-GGTGGCGGTGGAAGCGGCGGTGGCGGCGGAAGCGGCGGTGGCGGCAGCATGAGCACGAATCCTAAACCTC -3’	621
	P4: 5’-ACTGGATATCTTAGGCT GAAGCGGGCAC AG-3’
HBV X-HCV C fusion gene	P1: 5’-ATCTGTCGACATGGCTGCTAGGCTGTGCT G-3’	1086
	P4: 5’-ACTGGATATCTTAGGCTGAAGCGGGCAC AG-3’
c-myc	5’-TTCGGGTAGTGGAAAACCAG-3’	203
	5’-CAGCAGCTCGAATTTCTTCC-3’
β-actin	5’-GTGGGGCGCCCCAGGCACCA-3’	540
	5’CTTCCTTAATGTCACGCACGATTTC-3’

Table 2 Cell cycle of HepG2 cells infected with recombinant adenovirus (%, mean ± SE)

	G0/G1	S	G2/M
HepG2	71.57 ± 0.79	19.18 ± 0.77	9.25 ± 0.76
Ad0	72.94 ± 1.84	18.38 ± 0.9	8.68 ± 1.12
Ad-C	50.21 ± 1.37	32.15 ± 0.15b	17.65 ± 1.22b
Ad-X	48.17 ± 1.13	33.19 ± 1.47b	18.64 ± 0.56b
Ad-XC	36.49 ± 0.84	42.06 ± 0.24ab	21.45 ± 0.89ab

^aP < 0.005 vs Ad-C and Ad-X cells;

^bP < 0.001 vs HepG2 and Ad0 cells.