Effect of mutant p27kip1 gene on human cholangiocarcinoma cell line, QBC939

Table 1 In different testing groups QBC₉₃₉ cells

Group	Cell clone count (ratio, cell clone amounts/500)
	3 d after transfect	6 d after transfect	9 d after transfect	12 d after transfect
Ad-p27mt group	9 (1.8)	14 (2.8)	15 (3.0)	21 (4.2)
Ad-LacZ group (n = 3)	16 (3.2)	27 (5.4)	49 (9.8)	56 (11.2)
QBC939 group (n = 3)	18 (3.6)	31 (6.2)	46 (9.2)	62 (12.4)

F = 10.361, P = 0.011 (cell clone ratio, Ad-p27m vs AdLacZ group).

Table 2 Influence of gene transfect on QBC₉₃₉ cells at G₀/G₁ phase and apoptosis in different testing groups (mean ± SD)

Group	G0/G1 (%)/apoptosis (%)
	24 h after transfect	48 h after transfect	72 h after transfect
Ad-p27mt group (n = 3)	61.02 ± 1.03/ 0.81 ± 0.052	73.32 ± 2.99/ 5.27 ± 0.030	83.63 ± 2.10/ 11.61 ± 1.23
Ad-LacZgroup (n = 3)	54.91 ± 2.32/ 0.76 ± 0.031	62.56 ± 2.71/ 1.28 ± 0.043	63.57 ± 2.32/ 4.16 ± 0.230
QBC939 group (n = 3)	49.76 ± 1.97/ 0.78 ± 0.041	56.95 ± 1.06/ 1.10 ± 0.071	62.56 ± 2.88/ 4.13 ± 0.454

F = 15.954, P = 0.012 (G₀/G₁ cell ratio, Ad-p27m vs AdLacZ group); F = 3.236, P > 0.05 (apoptosis rates, Ad-p27m vs AdLacZ group).