Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4

Table 1 Effects of viable LBG, LBG-_S and H pyloriSS1-LPS on the expression of TLR4 and activation of TAK1, p38MAPK and NF-κB in SGC-7901 cells

	LPS 0 min	LPS 30 min	LPS 60 min	LPS 120 min		LBG + LPS 0 min	LBG + LPS 30 min	LBG + LPS 60 min	LBG + LPS 120 min	LBG-S +LPS 0 min	LBG-S + LPS 30 min	LBG-S + LPS 60 min	LBG-S + LPS 120 min
TLR4/β-actin	0.014 ± 0.003	0.21 ± 0.031	0.43 ± 0.051	1.21 ± 0.111		0.016 ± 0.003	0.08 ± 0.012	0.15 ± 0.022	0.40 ± 0.052	0.016 ± 0.003	0.088 ± 0.0132	0.12 ± 0.022	0.18 ± 0.032
1t		2.588	3.068	3.502	2t		2.328	2.425	2.302		2.182	2.733	2.901
1P		0.012	0.003	< 0.001	2P		0.031	0.021	0.033		0.041	0.01	0.007
p-TAK1/TAK1	0.008 ± 0.002	0.075 ± 0.0091	0.17 ± 0.031	0.49 ± 0.061		0.008 ± 0.001	0.025 ± 0.0032	0.051 ± 0.0062	0.15 ± 0.022	0.007 ± 0.002	0.031 ± 0.0052	0.086 ± 0.0112	0.13 ± 0.022
1t		2.445	2.871	3.421	2t		2.408	2.502	2.530		2.278	2.108	2.682
1P		0.018	0.005	< 0.001	2P		0.022	0.018	0.017		0.035	0.044	0.012
p-p38MAPK/p38MAPK	0.010 ± 0.002	0.079 ± 0.0111	0.18 ± 0.031	0.48 ± 0.081		0.009 ± 0.002	0.031 ± 0.0042	0.058 ± 0.0082	0.16 ± 0.032	0.013 ± 0.003	0.029 ± 0.0042	0.079 ± 0.0122	0.12 ± 0.022
1t		2.401	2.819	3.403	2t		2.261	2.445	2.506		2.289	2.093	2.704
1P		0.019	0.006	< 0.001	2P		0.036	0.02	0.018		0.034	0.046	0.011
NF-κB/lamin B1	0.012 ± 0.002	0.18 ± 0.031	0.32 ± 0.051	1.02 ± 0.131		0.012 ± 0.002	0.042 ± 0.0052	0.084 ± 0.0092	0.26 ± 0.042	0.014 ± 0.002	0.091 ± 0.0122	0.17 ± 0.022	0.23 ± 0.042
1t		2.523	2.968	3.458	2t		2.796	2.690	2.711		2.088	2.068	2.787
1P		0.014	0.004	< 0.001	2P		0.009	0.012	0.011		0.046	0.048	0.009

¹t and P value of each group vs corresponding LPS 0 min group;

²t and P value of each group vs corresponding LPS group at the same time point.

Table 2 Inhibitory effects of viable LBG or LBG-_S on H pyloriSS1-LPS-induced IL-8 production (pg/mL)

		H pyloriSS1-LPS		Viable LBG+LPS	LBG-S+LPS
0 min		2.62 ± 0.43		2.56 ± 0.46	2.52 ± 0.51
30 min		2.78 ± 0.38		2.67 ± 0.42	2.61 ± 0.47
60 min		3.07 ± 0.35		2.93 ± 0.51	2.89 ± 0.48
120 min		40.39 ± 3.01		24.12 ± 3.0512	18.41 ± 1.8312
	1t	2.832		2.601	2.506
	1P	0.006		0.011	0.015
			2t	2.121	2.175
			2P	0.047	0.044

¹t and P value of each group vs H pyloriSS1-LPS group at 0 min;

²t and P value of each group vs H pyloriSS1-LPS group at 120 min.