Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation

Table 1 Patient demographic, clinical and operative details

Parameters	n
Age (yr; mean ± SD, range)	59 ± 11 (33-78)
Male	10
Female	8
Primary tumor
Colorectal cancer	12
Benign liver lesions	2
Renal carcinomas	1
Prostate carcinoma	1
Cholangiocarcinoma	1
Pseudopapillary pancreas tumor	1
Operation
Right hemi-hepatectomy	7
Left hemi-hepatectomy	4
Extended right hemi-hepatectomy	2
Right lateral sectorectomy	2
Extended left hemi-hepatectomy	1
Left lateral sectorectomy	1
Non-anatomic resection	1

Table 2 Mice & cell transplantation details

Treatment groups	Mouse group	IP injection	No. of mice
Negative control (Hepatocyte only)	1	5 x 106 hepatocytes	3
Positive control 1 (HT-29 only)	2	5000 HT-29 cells	3
	3	100 000 HT-29 cells	3
	4	500 000 HT-29 cells	3
	5	2 million HT-29 cells	3
Positive control 2 (Hepatocytes + HT-29)	6	5 million hepatocytes + 5000 HT-29	3
	7	5 million hepatocytes + 100 000 HT-29	3
	8	5 million hepatocytes + 500 000 HT-29	3
	9	5 million hepatocytes + 2 million HT-29	3
Bead (Hepatocyte + HT-29)	10	5 million hepatocytes + 5000 HT-29	3
	11	5 million hepatocytes + 100 000 HT-29	3
	12	5 million hepatocytes + 500 000 HT-29	3
	13	5 million hepatocytes + 2 million HT-29	3

Table 3 Tumor growth

Treatment groups	IP injection	Mouse with tumour	Percentage expression (%)
Negative control (Hepatocyte only)	5 x 106 hepatocytes	0/3	0
Positive control 1 (HT-29 only)	5000 HT-29 cells	0/3	0
	100 000 HT-29 cells	1/31	01
	500 000 HT-29 cells	2/3	67
	2 million HT-29 cells	3/3	100
Positive control 2 (Hepatocytes + HT-29)	5 million hepatocytes + 5000 HT-29	0/3	0
	5 million hepatocytes + 100 000 HT-29	0/3	0
	5 million hepatocytes + 500 000 HT-29	3/3	100
	5 million hepatocytes + 2 million HT-29	2/3	67
Bead (Hepatocyte + HT-29)	5 million hepatocytes + 5000 HT-29	0/3	0
	5 million hepatocytes + 100 000 HT-29	0/3	0
	5 million hepatocytes + 500 000 HT-29	0/3	0
	5 million hepatocytes + 2 million HT-29	0/3	0

¹Injection-site tumor in skin only; In italics：Groups in which tumor was found; In standard form: Tumor-free.