Diversity of Helicobacter pylori isolates in expression of antigens and induction of antibodies

Table 1 Major information relative to PCR for amplifying 7 genes of H pylori

Target genes	Primer sequences (5’-3’)	Reference	Tm (°C)	Products (bp)
ureB	F: CGTCCGGCAATAGCTGCCATAGT	[18]	55	463
	R: GTAGGTCCTGCTACTGAAGCCTTA
hpaA	F: CTTTTAGGTGCGAGCGTGGTG	[19]	54	716
	R: AATTCCTTGGCGTCTTTTTGATAA
vacA	F: ATGGAAATACAACAAACACACCG	[20]	54	337
	R: CAACCTCCATCAATCTTACTGGA
CagA	F: ATAAGTCTAAATTAGACAACTTGAGCGA	[21]	56	297
	R: TTAGAATAATCAACAAAC ATCACGCCAT
napA	F: TGCGATCGTGTTGTTTATG	[22]	50	344
	R: GATCGTCCGCATAAGTTAC
flaA	F: GCTTTTTCAGGTCAATAC	[13]	52	524
	R: GTAGCGATACGAACCTGA
flaB	F: GATAAATACCAATATCGC	[13]	52	241
	R: CTCATCCATCGCTTTATC

Table 2 Comparison between positive rates of 7 genes and their products of H pylori and serum antibodies

Target gene (positive/negative)	Positive rate (%)	Target antigen (positive/negative)	Positive rate (%)	Target antibody (positive/negative)	Positive rate (%)
ureB (151/0)	100	UreB (151/0)	100	Anti-UreB (151/0)	100
hpaA (151/0)	100	HpaA (151/0)	100	Anti-HpaA (132/19)	87.4
vacA (151/0)	100	VacA (79/72)	52.3	Anti-VacA (65/86)	43.0
CagA (146/5)	96.7	CagA (139/12)	92.1	Anti-CagA (108/43)	71.5
napA (148/3)	98.0	NapA (141/10)	93.4	Anti-NapA (135/16)	89.4
flaA (151/0)	100	FlaA (151/0)	100	Anti-FlaA (128/23)	84.8
flaB (151/0)	100	FlaB (151/0)	100	Anti-FlaB (120/31)	79.5

Table 3 Correlation between positive H pylori infection rates and virulent factor expression in different gastric diseases

Disease	Patient	Positive number/rate of bacteria, n/%	Positive number/rate of virulence factor, n/%
			UreB	VacA	CagA	NapA
Chronic gastritis	240	102/42.5	102/100	47/46.1a	93/91.2	94/92.2
Surface gastritis	93	38/40.9	38/100	13/34.2	34/89.5	36/94.7d
Active gastritis	120	52/43.3	52/100	30/59.6c	48/92.3	50/96.2d
Atrophic gastritis	27	12/44.4	12/100	4/33.3	11/91.7	8/66.7
Peptic ulcer	92	43/46.7	43/100	29/67.4	40/93.0	41/95.4
Stomach	35	16/45.7	16/100	11/68.9	15/93.8	15/93.8
Duodenum	39	18/46.2	18/100	11/61.1	16/88.9	17/94.4
Complexity	18	9/50.0	9/100	7/77.8	9/100	9/100
Gastric adenocarcinoma	15	6/40.0	6/100	3/50.0	6/100	6/100

^aP < 0.05 vs peptic ulcer,

^cP < 0.05 vs active gastritis,

^dP < 0.01 vs atrophic gastritis.