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©2008 The WJG Press and Baishideng.
World J Gastroenterol. Jan 21, 2008; 14(3): 479-483
Published online Jan 21, 2008. doi: 10.3748/wjg.14.479
Published online Jan 21, 2008. doi: 10.3748/wjg.14.479
Table 1 Sensitivity of the Fosun real-time PCR HBV DNA assay and results of interassay testing
NICPBP references | Reference range (copies/mL) | Results (copies/mL) | Mean (log 10 copies/mL) | SD (log 10 copies/mL) | CV (%) | ||
Run 1 | Run 2 | Run 3 | |||||
L0 | 7.76 × 107 - 6.17 × 108 | 1.65 × 108 | 1.84 × 108 | 1.52 × 108 | 8.22 | 0.04 | 0.5 |
L1 | 1.48 × 107 - 1.18 × 108 | 9.21 × 107 | 1.01 × 108 | 9.23 × 107 | 7.98 | 0.02 | 0.3 |
L2 | 1.59 × 106 - 1.26 × 107 | 8.54 × 106 | 9.00 × 106 | 9.20 × 106 | 6.95 | 0.02 | 0.3 |
L3 | 1.66 × 105 - 1.32 × 106 | 7.06 × 105 | 6.13 × 105 | 9.55 × 105 | 5.87 | 0.10 | 1.7 |
L4 | 1.82 × 104 - 1.48 × 105 | 8.60 × 104 | 3.84 × 104 | 6.30 × 104 | 4.77 | 0.18 | 3.8 |
L5 | 1.51 × 103 - 1.23 × 104 | 1.07 × 104 | 6.20 × 103 | 8.82 × 103 | 3.92 | 0.12 | 3.1 |
L6 | < 1000 | 524 | 442 | 581 | 2.71 | 0.06 | 2.2 |
Table 2 Intra-assay variation of the Fosun real-time PCR HBV DNA assay
No. of samples | Replicates | Mean of HBV DNA | SD | CV (%) |
level (log10 copies/mL) | ||||
3768 | 12 | 3.97 | 0.32 | 8.1 |
3785 | 12 | 6.00 | 0.12 | 2.0 |
3770 | 12 | 7.84 | 0.11 | 1.4 |
- Citation: Shi M, Zhang Y, Zhu YH, Zhang J, Xu WJ. Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. World J Gastroenterol 2008; 14(3): 479-483
- URL: https://www.wjgnet.com/1007-9327/full/v14/i3/479.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.479