Replication of clinical hepatitis B virus isolate and its application for selecting antiviral agents for chronic hepatitis B patients

Table 1 HBsAg and HBeAg levels of clinical HBV isolates in culture supernatants (mean ± SD)

	24 h		48 h		72 h		96 h
Isolates	HBsAg	HBeAg	HBsAg	HBeAg	HBsAg	HBeAg	HBsAg	HBeAg
1	0.236 ± 0.028	0.158 ± 0.018	0.792 ± 0.055	0.435 ± 0.039	1.892 ± 0.168	1.276 ± 0.138	1.725 ± 0.258	1.212 ± 0.237
2	0.225 ±0.019	0.149 ± 0.014	0.646 ± 0.043	0.453 ± 0.044	1.737 ± 0.132	1.321 ± 0.215	1.722 ± 0.633	1.121 ± 0.153
3	0.214 ± 0.038	0.152 ± 0.015	0.756 ± 0.075	0.421 ± 0.054	1.654 ± 0.156	1.232 ± 0.342	1.563 ± 0.742	1.133 ± 0.217
4	0.195 ± 0.022	0.122 ± 0.012	0.533 ± 0.057	0.349 ± 0.033	1.278 ± 0.132	1.133 ± 0.142	1.236 ± 0.571	1.123 ± 0.342
5	0.189 ± 0.028	0.112 ± 0.013	0.636 ± 0.062	0.352 ± 0.024	0.996 ± 0.114	0.982 ± 0.211	1.006 ± 0.263	1.012 ± 0.411
6	0.188 ± 0.033	0.132 ± 0.013	0.537 ± 0.075	0.384 ± 0.042	0.987 ± 0.132	0.952 ± 0.156	0.945 ± 0.154	0.943 ± 0.125
7	0.179 ± 0.018	0.122 ± 0.012	0.639 ± 0.031	0.298 ± 0.027	1.139 ± 0.163	0.892 ± 0.142	0.997 ± 0.218	0.885 ± 0.218
8	0.236 ± 0.043	0.155 ± 0.015	0.836 ± 0.061	0.475 ± 0.039	1.754 ± 0.174	1.312 ± 0.111	1.825 ± 0.318	1.212 ± 0.323
Positive	0.336 ± 0.048	0.217 ± 0.028	0.956 ± 0.078	0.552 ± 0.066	1.868 ± 0.218	1.202 ± 0.324	1.922 ± 0.525	1.189 ± 0.305
Negative	0.046 ± 0.012	0.032 ± 0.011	0.044 ± 0.018	0.040 ± 0.015	0.047 ± 0.020	0.038 ± 0.012	0.042 ± 0.013	0.039 ± 0.018

Plasmid pHBV 1.3 is positive control; Plasmid pHY106 is negative control.

Table 2 Viral loads in supernatants of Huh7 cells transfected with HBV replicon (copies/mL)

Replicons	Transfected time (h)
	24	48	72	96
1	5.6 × 102	2.5 × 103	3.6 × 105	3.9 × 105
2	5.2 × 102	2.0 × 103	2.8 × 105	2.7 × 105
3	4.5 × 102	1.8 × 103	2.4 × 105	2.6 × 105
4	< 102	1.1 × 103	7.5 × 104	9.7 × 104
5	< 102	1.2 × 103	8.2 × 104	9.1 × 104
6	< 102	1.5 × 103	7.8 × 104	8.9 × 104
7	< 102	1.3 × 103	9.5 × 104	1.2 × 105
8	5.5 × 102	2.3 × 103	2.8 × 105	2.9 × 105
pHBV1.3	6.5 × 102	2.8 × 103	2.5 × 105	2.7 × 105
pHY106	< 102	< 102	< 102	< 102

pHBV1.3 is positive control; pHY106 is negative control.