Relationship between expression of gastrin, somatostatin, Fas/FasL and caspases in large intestinal carcinoma

Table 1 Primers used for nested RT-PCR amplification of GAS and SS

Name	Primer sequence	PCR conditions	Size (bp)
GAS	1: 5’-TATGTGCTGATCTTTGCACTGGCT-3’ (sense: 6307-6330)	94°C, 30 s	282
	2: 5’-CTCATCCTCAGCACTGCGGCGGCC-3’ (antisense: 6718-6695)	60°C, 45 s
		72°C, 45 s
GAS	3: 5’-GAGCTACCCTGGCTGGAGCAGCAG-3’ (sense: 6415-6438)	94°C, 30 s	174
	2: 5’-CTCATCCTCAGCACTGCGGCGGCC-3’ (antisense: 6718-6695)	60°C, 45 s
		72°C, 45 s
SS	1: 5’-ATGCTGTCCTGCCGCCTCCAG-3’ (sense: 106-126)	94°C, 30 s	348
	2: 5’-ACAGGATGTGAAAGTCTTCCA-3’ (antisense: 1330-1310)	60°C, 45 s
SS	3: 5’-GCTGCTGCCGCGGGGAAGCAG-3’ (sense: 223-243)	94°C, 30 s	231
	2: 5’-ACAGGATGTGAAAGTCTTCCA-3’ (antisense: 1330-1310)	60°C, 45 s
		72°C, 45 s

Table 2 Correlation between clinicopathological factors and GAS and SS mRNA and protein expression in LIC

Groups	n	mRNA						Protein
		GAS (+)	χ2	P	SS (+)	χ2	P	GAS (+)	χ2	P	SS (+)	χ2	P
Differentiation
Well	27	8b	10.47	< 0.01	18			6b	10.23	< 0.01	17	5.24	< 0.05
Moderate	28	11a	6.68	< 0.05	10c		< 0.05	10a	4.95	< 0.05	9c	11.24	< 0.01
Poor	24	18			5d	10.78	< 0.01	16			4d
Histological types
Papillary	19	6e	4.80	< 0.05	13			4e	6.22	< 0.05	12
Tubular	35	13e	4.40	< 0.05	14			11e	4.38	< 0.05	14
Mucinous and signet-ring	16	11			4g	6.56	< 0.05	10			3g	6.99	< 0.05
Undifferentiated	9	7			2g	5.44	< 0.05	7			1g	7.33	<0.05
Dukes’ stage
A and B	36	12i	4.84	< 0.05	20i	5.17	< 0.05	10i	4.44	< 0.05	18i	4.06	< 0.05
C and D	43	25			13			22			12

^aP < 0.05,

^bP < 0.01 vs poor differentiation;

^cP < 0.05,

^dP < 0.01 vs well differentiation;

^eP < 0.05 vs mucinous and signet-ring and undifferentiated;

^gP < 0.05 vs papillary;

ⁱP < 0.05 vs Dukes’ stages C and D.

Table 3 Comparison of AI in GAS and SS high, moderate and low expression groups of LIC (mean ± SD)

Groups	n	A I (mean ± SD)	F	P
SS
Low	10	5.35 ± 3.00
Moderate	20	7.44 ± 2.67b	17.63	< 0.01
High	49	9.03 ± 1.76b
GAS
Low	47	7.82 ± 2.38
Moderate	21	5.51 ± 2.66d	16.08	< 0.01
High	11	3.75 ± 2.38d

^bP < 0.01 vs low SS group;

^dP < 0.01 vs low GAS group.

Table 4 Comparison of positive rates of Fas, FasL, caspase-3 and caspase-8 protein in GAS and SS high, moderate and low expression groups of LIC

Groups	n	Fas		FasL		Caspase-3		Caspase-8
		+ (%)	-	+ (%)	-	+ (%)	-	+ (%)	-
GAS
High	11	7 (63.6)	4	10 (90.9)a	1	9 (81.8)	2	8 (72.7)	3
Moderate	21	16 (76.2)	5	7 (81.0)a	4	14 (66.7)	7	13 (61.9)	8
Low	47	37 (78.7)	10	26 (53.2)	21	29 (61.7)	18	28 (59.6)	19
SS
High	10	9 (90.0)c	1	8 (80.0)	2	10 (100.0)c	0	9 (90.0)c	1
Moderate	20	16 (80.0)c	4	14 (70.0)	6	15 (75.0)c	5	14 (70.0)c	6
Low	49	26 (53.1)	23	32 (65.3)	17	24 (49.0)	25	21 (42.9)	28

^aP < 0.05 vs GAS low expression group;

^cP < 0.05 vs SS low expression group.