Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca2+-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats

Table 1 Probe, primer and product (bp) in RT-PCR

Genes	Probes	Upstream primers	Downstream primers	Product (bp)
SERCA1	5’-CGATGTCCCGAGCCTTGATCC-3’	5’-GGTTTGGCAGGAACGGAAT-3’	5’-GGTGGATTTGATGGAGAGGAT-3’	199
SERCA2	5’-CACACTCTTTCTGTCCTGTCG-3’	5’-ATGAACCTGAAATGGGCAAG-3’	5’-GGAACTTTGTCACCAACAGCA-3’	115
GAPDH	5’-FAM-ACCACAGTCCATGCCATCAC-TAMRA-3’	5’-CCTCAAGATTGTCAGCAAT-3’	5’-CCATCCACAGTCTTCTGAGT-3’	141

SERCA: Sarco/endoplasmic reticulum Ca²⁺-ATPase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 2 Pathological scoring criteria for pancreatic tissues of AP rats

Pathological changes	Scores
Edema
Focal expansion of interlobular septa	1
Same as 1 + diffuse expansion of interlobar;	2
Septa/diffuse expansion of interlobar septa
Same as 2 + expansion of interacinar septa	3
Same as 3 + expansion of intercellular spaces	4
Inflammation and perivascular infltrate
2-10 intralobular or perivascular leukocytes/HPF	1
11-20 intralobular or perivascular leukocytes/HPF	2
21-30 intralobular or perivascular leukocytes/HPF	3
> 30 leukocytes/HPF or confluent microabscesses	4
Acinar necrosis
Diffuse occurrence of 1-4 necrotic cells/HPF	1
Diffuse occurrence of 5-10 necrotic cells/HPF	2
Diffuse occurrence of 11-16 necrotic cells/HPF (foci of confluent necrosis)	3
> 16 necrotic cells/HPF (Extensive confluent necrosis)	4
Hemorrhage and fat necrosis
1-2 focus	1
3-4 focus	2
5-6 focus	3
> 7 focus	4