Enhancement of CD4+ T cell activities and modulation of Th1/Th2 lineage development in radiated tumor-bearing rats treated with male zooid of Antheraea pernyi extracts

Table 1 Effect of MZAPE on tumor weight, tumor-inhibiting rate, suppression of anti-HEL IgG antibody production, and proliferative responses of PBMC to HEL (n = 20, mean ± SE)

Groups	Tumor weight (g)	Tumor-inhibiting rate (%)	Anti-HEL IgG antibody (A405)	Proliferation (× 1000 cpm)
Control	4.27 ± 0.60		0.17 ± 0.02	6.0 ± 0.7
Simple irradiation	1.58 ± 0.40b	63.08 ± 6.43	0.09 ± 0.01b	5.31 ± 0.8d
Radiation plus MZAPE	1.29 ± 0.33b	69.86 ± 7.12	0.34 ± 0.01b	27.4 ± 2.8b
MZAPE	2.76 ± 0.37b	35.30 ± 7.67	0.44 ± 0.04b	49.6 ± 3.1b

^bP < 0.001 and

^dP = 0.006 vs control group.

Table 2 Effect of MZAPE on anti-HEL IgG2a and IgG1 antibody production (n = 20, mean ± SE)

Groups	Anti-HEL IgG2a antibody (A405)	P	Anti-HEL IgG1 antibody (A405)	P
Control	0.08 ± 0.01		0.25 ± 0.02
Simple irradiation	0.06 ± 0.01	< 0.001	0.51 ± 0.03	< 0.001
Radiation plus	0.15 ± 0.02	< 0.001	0.396 ± 0.02	< 0.001
MZAPE group
MZAPE group	0.32 ± 0.04	< 0.001	0.24 ± 0.01	0.053

Table 3 Effect of MZAPE on peripheral blood CD4 and CD8 cells (n = 20, mean ± SE)

	Lymphocyte subgroups (%)
	CD4+ (%)	P	CD8+ (%)	P	CD4/CD8	P
Control group	38.05 ± 5.05		19.30 ± 2.17		1.97 ± 0.35
Simple irradiation group	30.36 ± 2.12	< 0.001b	17.70 ± 2.34	0.031	1.72 ± 0.23	0.011
Radiation plus MZAPE group	36.99 ± 5.24	0.519	17.30 ± 3.44	0.034	2.13 ± 0.32	0.140
MZAPE group	32.13 ± 3.85	< 0.001b	16.70 ± 1.82	< 0.001b	1.92 ± 0.26	0.611

^bP < 0.001 vs control group.

Table 4 Effect of MZAPE on Th1 and Th2 cytokines (n = 20, mean ± SE) (pg/mL)

	IL-2	P	INF-γ	P	IL-4	P	IL-10	P
Control group	181.99 ± 19.33		89.06 ± 21.44		97.69 ± 7.11		180.14 ± 19.56
Simple irradiation group	129.14 ± 16.96	< 0.001b	74.09 ± 39.29	0.143	100.57 ± 12.01	0.362	179.26 ± 18.13	0.883
Radiation plus	138.16 ± 23.15	< 0.001b	91.25 ± 27.00	0.778	74.78 ± 8.28d	< 0.001bd	180.25 ± 17.32	0.985
MZAPE group
MZAPE group	166.73 ± 13.34d	0.006b	103.85 ± 6.55d	0.005b	66.02 ± 7.14d	< 0.001bd	136.29 ± 18.21d	< 0.001bd
		< 0.001d		0.002d

^bP < 0.001 vs control group;

^dP < 0.001 vs simple irradiation group.