Pancreatic stellate cells promote proliferation and invasiveness of human pancreatic cancer cells via galectin-3

Table 1 PSC cycle detected by flow cytometry (%)

Group	Medium	G0 + G1 fraction	S phase fraction	G2/M fraction	S + G2/M fraction
1 (Control)	Serum-free medium	89.81	1.94	8.25	10.19
2	Serum-free medium containing 40% SSW	89.63	3.23	7.14	10.37
3	Serum-free medium containing 40% SSW and 1 &mgr;g/mL GAL-3 antibody	89.42	2.70	7.88	10.58
4	Serum-free medium containing 100 ng/mL GAL-3 protein	87.04	3.68	9.28	12.96

Group 1: PSCs exposed to serum-free medium (control group); Group 2: PSCs exposed to serum-free medium containing 40% SSW; Group 3: PSCs exposed to serum-free medium containing 40% SSW and 1 &mgr;g/mL GAL-3 antibody; Group 4: PSCs exposed to serum-free medium containing 100 ng/mL GAL-3 protein.

Table 2 Proliferation of SW1990 cells detected by flow cytometry

Group	Medium	G0 + G1 fraction	S phase fraction	G2/M fraction	S + G2/M fraction
1 (control)	Serum-free DMEM	71.80%	16.76%	11.45%	28.20%
2	Serum-free DMEM containing 1 &mgr;g/mL GAL-3 MA	79.28%	13.87%	6.85%	20.72%
3	Serum-free DMEM containing 40% SPSC	62.51%	20.34%	17.15%	37.49%
4	Serum-free DMEM containing 40% SPSC and 1 &mgr;g/mL GAL-3 MA	67.91%	20.56%	11.53%	32.09%

Group 1: Sw1990 cells exposed to serum-free DMEM (control group); Group 2: SW1990 cells exposed to serum-free DMEM containing 1 &mgr;g/mL GAL-3 MA; Group 3: PSCs exposed to serum-free DMEM containing 40% SPSC; Group 4: PSCs exposed to serum-free DMEM containing 40% SPSC and 1 &mgr;g/mL GAL-3 MA.