Refined mapping of loss of heterozygosity on 1q31.1-32.1 in sporadic colorectal carcinoma

Table 1 The primer sequences of seven microsatellite markers

Primer	3’-5’	5’-3’
D1S2877	AGACATTNCATTGAAGTCTATTTAT	CAAGCCACTAGCGTAAGAGC
D1S412	TAGGACTTTTCAACTTCCACAG	ATAGGCACAGAATCAATGAATG
D1S2757	TTTTTTAATGACTGACCAGTG	TGCCTTCTGCTATGTTTG
D1S413	GCCAAGCCTGAGATCAAAAT	ACTTGAACAGATTGGGATTG
D1S2622	CTGCAACATAAGAACCTAGTGTAAC	AAACTGGTAGGCCATTGATAGA
D1S2683	TGCCTTGTCTTCAAGAGC	GCAGTGACAGGAATCTGG
D1S2668	AATCACTTGAACCTGGGAG	ACTGACTGGCTGTTTCTGAG

Table 2 LOH frequencies of microsatellite loci on 1q31.1-32.1 in colorectal cancer

Locus	Location	LOH case	Normal case	LOH rate (%)	Informative rate (%)
D1S2877	1q31.1	7	29	19.44	43.37
D1S412	1q31.3	11	56	16.42	80.72
D1S2757	1q31.3	18	43	29.51	73.49
D1S413	1q31.3	13	27	32.50	48.19
D1S2622	1q32.1	18	31	36.73	59.04
D1S2683	1q32.1	10	41	19.61	61.45
D1S2668	1q32.1	4	20	16.67	28.92

Table 3 The relationship between clinicopathological features and LOH of the loci on 1q31.1-32.1

		D1S2877		D1S412		D1S2757		D1S413		D1S2622		D1S2683		D1S2668
		T1	N2	T	N	T	N	T	N	T	N	T	N	T	N
Gender	Male	4	13	3	27	10	18	7	14	11	15	5	20	2	5
	Female	3	16	8	29	8	25	6	13	7	16	5	21	2	15
Age(yr)	> 60	5	24	9	39	13	32	10	18	16	22	10	29	2	16
	≤ 60	2	5	2	17	5	11	3	9	2	9	0	12	2	4
Location	Proximal colon	2	10	6	22	5	18	6	10	9	13	6	13	0	9
	Distal colon	2	8	4	15	5	11	3	7	5	8	0	14	1	7
	Rectum	3	11	1	19	8	14	4	10	4	10	4	14	3	4
Gross pattern	Massive	2	15	5	25	7	22	5	14	6	16	3	22	1	9
	Ulcerative	4	13	5	23	9	18	5	9	9	14	6	17	1	7
	Encroaching	1	1	1	8	2	3	3	4	3	1	1	2	2	4
Size (cm)	≥ 5	4	12	7	22	6	18	6	11	8	15	5	17	2	9
	< 5	3	17	4	34	12	25	7	16	10	16	5	24	2	11
LN metastasis	(+)	4	20	6	39	12	29	6	16	15	20	5	26	1	16
	(-)	3	9	5	17	6	14	7	11	3	11	5	15	3	4
Differentiation	Well	3	4	1	14	5	7	3	7	4	5	1	10	3	5
	Moderately	2	21	8	26	8	27	6	12	10	18	5	25	0	12
	Poorly	1	1	2	2	2	2	1	2	1	2	2	1	0	1
	Mucinous	1	3	0	14	3	7	3	6	3	6	2	5	1	2
Dukes stage	A	2	5	2	3	3	3	2	3	1	3	2	5	0	1
	B	1	4	3	14	3	11	5	8	2	8	3	10	3	3
	C	3	16	4	29	11	19	4	12	10	17	3	19	0	13
	D	1	4	2	10	1	10	2	4	5	3	2	7	1	3

T: Tumor; N: Normal.