Genetic changes of p53, K-ras, and microsatellite instability in gallbladder carcinoma in high-incidence areas of Japan and Hungary

Table 1 Genetic alterations observed in gallbladder carcinomas from Japan and Hungary

Case No	Age (yr)	Sex	Histology1		p53 mutation		K-ras mutation	Microsatellite instability
			Type	Grade	Exon/codon	Base change	Base change
Japanese cases
1	71	F	PAP	G1	None	-	None	BAT25, BAT26
2	74	F	AD	G1	Exon 5/codon 132	AAG to GAG	None	BAT25, D2S123
3	62	F	PAP	G1	None	-	None	BAT25
4	76	F	AD	G1	Exon 6/codon 193	CAT to AAT	None	BAT25, BAT26
5	78	F	AD	G2	Exon 5/codon 140	ACC to ATC	None	ND
					Exon 5/codon 166	TCA to ACA
6	70	F	AD	G1	Exon 8/codon 276	GCC to CCC	None	None
7	71	F	AD	G1	None	-	None	BAT26, D2S123
8	79	F	AD	G1	None	-	None	None
9	66	F	PAP	G1	None	-	None	None
10	60	F	PAP	G1	None	-	None	None
11	80	M	PAP	G1	None	-	None	BAT26
12	63	M	PAP	G1	Exon 8/codon 294	GAG to GAA	None	BAT25, BAT26
13	49	F	PAP	G1	None	-	None	D2S123
14	71	M	AD	G2	Exon 8/codon 280	AGA to AAA	None	BAT25, D2S123
15	70	M	AD	G1	Exon 7/codon 238	TGT to CGT	None	ND
16	53	M	AD	G1	None	-	None	None
17	79	F	AD	G3	Exon 8/codon 271	GAG to AAG	None	None
18	62	M	AD	G3	Exon 6/codon 205	TAT to TGT	None	None
19	61	M	AD	G1	None	-	None	D17S250, D2S123
20	69	M	AD	G2	Exon 7/codon 231	ACC to ATC	None	BAT25, D17S250
21	58	F	AD	G2	Exon 5/codon 160	ATG to GTG	None	ND
					Exon 6/codon 220	TAT to AAT
22	76	F	AD	G3	None	-	None	None
Hungarian cases
23	71	M	AD	G2	Exon 7/codon 249	AGG to ACG	None	BAT26
24	69	F	AD	G3	Exon 6/codon 213	CAG to CGG	None	None
25	69	F	AD	G2	None	-	None	None
26	76	F	AD	G2	None	-	None	None
27	61	F	AD	G3	None	-	None	None
28	58	F	AD	G3	None	-	None	BAT25
29	69	F	AD	G3	None	-	None	None
30	75	F	PAP	G1	None	-	None	None
31	66	F	AD	G3	None	-	None	None
32	65	F	AD	G1	Exon 6/codon 219	CCC to TCC	None	ND
33	61	F	AD	G3	ND	-	None	ND
34	Unknown	F	PAP	G1	Exon 5/codon 138	GCC to GCT	None	BAT25, D17S250
					Exon 5/codon 167	CAG to CAA
					Exon 5/codon 170	ACG to ACA
35	Unknown	F	PAP	G1	Exon 5/codon 158	CGC to TGC	GGT to AGT	ND
					Exon 6/codon 223	CCT to TCT
36	Unknown	F	AD	G3	Exon 6/codon 210	AAC to AAT	None	ND
					Exon 7/codon 228	GAC to AAC
37	Unknown	F	AD	G2	ND	-	None	ND
38	53	F	AD	G3	None	-	None	None
39	72	Unknown	AD	G1	None	-	None	None
40	69	M	AD	G1	None	-	None	BAT26
41	57	F	AD	G1	None	-	None	None
42	74	F	AD	G1	None	-	None	BAT25

¹According to the tumor-node-metastasis (TNM) staging system[18]. PAP: Papillary carcinoma; AD: Adenocarcinoma; ND: Not detected; G1: Well differentiated; G2: Moderately differentiated; G3: Poorly differentiated.

Table 2 Association between p53 mutation and MSI in gallbladder carcinoma

		MSI-high	MSI-low/none	P
53 mutation	Present	6	5	0.033
	Absent	3	20

MSI: Microsatellite instability.