Effects of endogenous nitric oxide induced by 5-fluorouracil and L-Arg on liver carcinoma in nude mice

Table 1 Changes of tumor volume and tumor inhibition rate

	n	Changes of tumor volume (mm3)	Tumor inhibition rate (%)
A: NS group	10	257.978 ± 59.0a
B: 5-FU group	10	172.232 ± 66.0c	33.24
C: 5-FU+L-Arg group	10	91.523 ± 26.7e	64.52

^aP < 0.05, group B vs C;

^cP < 0.05, A vs C;

^eP < 0.05, A vs B.

Table 2 Necrotic range in tumor tissues

	+	++	+++	++++
A: NS group	6	3	1
B: 5-FU group		5	3	2
C: 5-FU+L-Arg group		1	4	5

χ² = 15.963, P < 0.05.

Table 3 Optical density of iNOS ,iNOS activity and NO concentration in tumor tissues

	Optical density of iNOS (μmol/L)	Activity of iNOS (U/mg protein)	Concentrationof NO
A: NS group	0.4621 ± 0.0115a	4.87 ± 2.5a	6.58 ± 3.2a
B: 5-FU group	0.4783 ± 0.0107c	9.83 ± 2.31c	17.97 ± 6.16c
C: 5-FU+L-Arg group	0.5269 ± 0.0034e	15.1 ± 4.91e	30.41 ± 8.81e

F of optical density of iNOS , iNOS activity and NO concentration are 31.693, 21.949 and 33.909, respectively,

^aP < 0.05, B vs C;

^cP < 0.05, A vs C;

^eP < 0.05, A vs B.

Table 4 The optical density of P16 and BAX in tumor tissues

	Optical density of BAX	Optical density of P16
A: NS group	0.4501 ± 0.0114a	0.4565 ± 0.0139a
B: 5-FU group	0.4788 ± 0.0068c	0.4939 ± 0.041c
C: 5-FU+L-Arg group	0.5045 ± 0.0199e	0.5451 ± 0.027e

F of optical density of BAX andP16 is 23.51 and 16.13.

^aP < 0.05, B vs C;

^cP < 0.05, A vs C;

^eP < 0.05, A vs B.