Mycobacterium avium subspecies paratuberculosis infects and multiplies in enteric glial cells

Table 1 PCR primers, targets, position on the sequences

Target	Primer name	5'-3' sequence	Position1
16S	MAP16Sfor	ATCATGCCCCTTATGTCCAG	1179-1198
	MAP16Srev	TGAGACCGGCTTTAAAAGGA	1259-1278
sigA	sigAfor	GTACGCCACCCAGCTGATGTCG	714-735
	sigArev	CGTCGCGGCAGATCCACAT	787-805
sigB	sigBfor	GACCTGCTCGAGCACAGC	646-663
	sigBrev	CAGCACGCTGCGGATGTCGGTG	783-804
sigC	sigCfor	ACATCCGTCACCTGCAGTC	1088-1106
	sigCrev	GTCACCTCGACCAGATCCTC	1177-1195
sigD	sigDfor	CTTCCTGGCTTTCCTGTACG	249-268
	sigDrev	GATGGACTCGGTCGGGTAG	324-242
sigE	sigEfor	CACCCAGGAGACCTTCATCC	327-346
	sigErev	GACCATGTCCAGGAACAG	418-435
sigF	sigFfor	GGCAGCTCCTACAACACCTT	493-512
	sigFrev	ACTCCTGGTCCTCGATCCGG	597-616
sigF-like	sigF-likefor	ATGACCAACGCAATCGCTCC	1-20
	sigF-likerev	GGCATCCGGCGCAGTTCCA	88-107
sigG	sigGfor	GCGTTCGAAAGCTACGACAT	622-641
	sigGrev	CTGATACCACCCGGTGTACG	692-711
sigH	sigHfor	AATCTCAAGGCGTGGCTCTA	385-404
	sigHrev	TGATTTCCTCGGTCGGATAC	465-484
sigI	sigIfor	GGGCGACATCGACGACGTGC	150-169
	sigIrev	GACATCGCCCGGACGTTCGTG	235-255
sigJ	sigJfor	GCATCTACACGGCGGGCCTG	740-759
	sigJrev	GGCGAACCGGTGAACTTGT	857-875
sigL	sigLfor	CGTGATCGAACGGTCCTACT	399-418
	sigLrev	CCGCACCGCATAGTGTAGT	491-504
sigM	sigMfor	TGGCTGCACCGCATCGTG	238-255
	sigMrev	TCGGCGACCGGATAGTAGTCT	315-335
Other ECF-1	sigECF1for	GTTCTCCGCCGAGTCGATTT	310-328
	sigECF1rev	GTCGAATCCGAACACCTCAC	492-411
Other ECF-2	sigECF2for	GCATCCACACGATCGACAT	839-857
	sigECF2rev	GGTTGTGATGTTCCTGAACC	914-933
Other ECF-3	sigECF3for	GTCGGTCATGGGTTTCGT	780-797
	sigECF3rev	GCACCCAGCTCCAGTTTC	855-873
Other ECF-4	sigECF4for	GATCTCGTCGGCATCTCG	502-519
	sigECF4rev	TCCAATTCGTTTCGGAGATT	592-611
Other ECF-5	sigECF5for	GCAATTGACCCGTTCACC	945-962
	sigECF5rev	CTCCTCCAAAGCGGCTAAG	1020-1038
Other ECF-6	sigECF6for	TGCAAGGTAATTCGATCAAGG	419-439
	sigECF6rev	TCCCTCGTTGACCTGTGC	511-528

¹longest sequence available (primary and TIGR annotation). ECF: Extracitoplasmatic function; TIGR: The Institute for Genomic Research.