Crohn’s disease incidence evolution in North-western Greece is not associated with alteration of NOD2/CARD15 variants

Table 1 Demographic characteristics of IBD patients diagnosed during the period 1983-2005, in northwest Greece

	Crohn’s disease	Ulcerative colitis
Total numbers of patients	105	325
Region of origin
Ioannina	59	140
Arta	5	10
Preveza	2	36
Thesprotia	5	21
Corfu	33	113
Lefkada	1	5
Male: female (ratio)	66:39 (1.69)	208:117 (1.77)
Age at diagnosis (yr) (mean ± SD)	33.2 ± 12.9	45.7 ± 16.1
(range)	(18-70)	(18-93)
Men	33.4 ± 12.9	47.3 ± 15.1
Women	33.1 ± 13.2	42.9 ± 17.3
Follow up (mo) (mean ± SD)	92.9 ± 72.3	119.6 ± 73.8
(range)	(4-264)	(5-276)

Table 2 Evolution of genetic, demographic and clinical correlations of Crohn’s disease through the study period

Period	AnyCARD15mutation	M:Fratio	Meanage(yr)	CD:UCratio,cumulative	CD:UCratio, bregion ofIoannina	CD:UCratio,regionof Corfu	Perianal
Pre-1994	46.10%	1.4	30.4	0.23	0.26	0.2	13.80%
1995-1999	61.90%	3.4	35.4	0.32	0.46	0.3	27.20%
2000-2005	46.90%	1.55	33.1	0.61	> 1	0.5	31.30%
Total	51%	1.83	32.8	0.36	0.38	0.32	25.50%

Table 3 CARD15 variant allele frequencies

	Genotype			Mutant allelefrequency (%)	P; OR (95% CI)
Samples	-/-	-/+	+/+
R702W
CD	85	8	3	7.29	0.015; 3.85 (1.24-11.93)
UC	171	9	0	2.5	0.78; 1.26 (0.38-4.13)
Control	96	4	0	2
G908R
CD	83	13	0	6.77	0.70; 0.83 (0.39-1.78)
UC	156	24	0	6.66	0.61; 0.82 (0.42-1.58)
Control	84	16	0	8
Leu1007fsincC
CD	52	36	8	27.08	< 0.0001; 7.06 (3.46-14.37)
UC	166	9	5	5.27	1; 1.06 (0.48-2.32)
Control	90	10	0	5

-/-, homozygous wild-type; -/+, heterozygous; +/+, homozygous mutant. CD: Crohn’s disease; UC: Ulcerative colitis; OR: Odds Ratio; CI: Confidence Interval. The CARD15 gene variants determined in 96 CD patients (from 102 living CD patients out of 105, from our cohort 6 refused genetic test), 180 out of 300 living UC patients and 100 randomly chosen healthy blood donors.