Rapid Communication
Copyright ©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 14, 2007; 13(34): 4630-4635
Published online Sep 14, 2007. doi: 10.3748/wjg.v13.i34.4630
Table 1 Primers used in amplifying HAV genome
NameSequencesPosition
F1T71-TTTCCGGAGYCCCTCTTG22-39
R1M13R2-ATCTGCCAGAGACAGGA806-790
F2T7-GAGGTACTCAGGGGCATTTA690-709
R2M13R-GGATCCAAAGCAAAAGACA1567-1549
F3T7-AGATTTGGAGTTGCATGGATTAAC1430-1453
R3M13R-GGATCCTGAAACATCCA2342-2326
F4M13R-TTCAACAACAGTTTCTACAG2231-2250
R4T7-AGCTTCCTCCTCTGATCTA3098-3080
F5T7-GGGAGATAAGACAGATTCTACAT2861-2883
R5M13R-AGCTTCCACTCTTAACAAA3813-3795
F6M13R-GAATGCTTGGATTGTCTGG3634-3652
R6T7-GATCCATCCCAGTAATCTGA4549-4530
F7T7-ATCAATTGCATTGGCAAC4451-4468
R7M13R-GGATCCGCATCTAATTTAATC5278-5258
F8M13R-GCTGTGGGAGCTGCAGTTGG5124-5143
R8T7-GATCCATGGGACTCTTTCTA6027-6008
F9T7-GAGGAAATTCAATTCTTG5872-5889
R9M13R-GGATGGAGTTCCAGA6764-6750
F10T7-GGAGATGTYGGTCTTGAT6657-6674
R10M13R-AGAGGGGTCTCCGGGAATTTpolyA
3'RACECAAGAGGGGTCTCCGGGAATTTTTTTTTTTTTTTTTTTTpolyA
Table 2 Divergence of H2w and its progenies compared to reference strains in VP1/2A region (%)
H2wH2K5H2K10H2K15K2K20H2K25H2K30
HM17510.13.63.63.63.63.63.6
LA4.87.77.77.77.77.77.7