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©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 14, 2007; 13(34): 4630-4635
Published online Sep 14, 2007. doi: 10.3748/wjg.v13.i34.4630
Published online Sep 14, 2007. doi: 10.3748/wjg.v13.i34.4630
Table 1 Primers used in amplifying HAV genome
| Name | Sequences | Position |
| F1 | T71-TTTCCGGAGYCCCTCTTG | 22-39 |
| R1 | M13R2-ATCTGCCAGAGACAGGA | 806-790 |
| F2 | T7-GAGGTACTCAGGGGCATTTA | 690-709 |
| R2 | M13R-GGATCCAAAGCAAAAGACA | 1567-1549 |
| F3 | T7-AGATTTGGAGTTGCATGGATTAAC | 1430-1453 |
| R3 | M13R-GGATCCTGAAACATCCA | 2342-2326 |
| F4 | M13R-TTCAACAACAGTTTCTACAG | 2231-2250 |
| R4 | T7-AGCTTCCTCCTCTGATCTA | 3098-3080 |
| F5 | T7-GGGAGATAAGACAGATTCTACAT | 2861-2883 |
| R5 | M13R-AGCTTCCACTCTTAACAAA | 3813-3795 |
| F6 | M13R-GAATGCTTGGATTGTCTGG | 3634-3652 |
| R6 | T7-GATCCATCCCAGTAATCTGA | 4549-4530 |
| F7 | T7-ATCAATTGCATTGGCAAC | 4451-4468 |
| R7 | M13R-GGATCCGCATCTAATTTAATC | 5278-5258 |
| F8 | M13R-GCTGTGGGAGCTGCAGTTGG | 5124-5143 |
| R8 | T7-GATCCATGGGACTCTTTCTA | 6027-6008 |
| F9 | T7-GAGGAAATTCAATTCTTG | 5872-5889 |
| R9 | M13R-GGATGGAGTTCCAGA | 6764-6750 |
| F10 | T7-GGAGATGTYGGTCTTGAT | 6657-6674 |
| R10 | M13R-AGAGGGGTCTCCGGGAATTT | polyA |
| 3'RACE | CAAGAGGGGTCTCCGGGAATTTTTTTTTTTTTTTTTTTT | polyA |
Table 2 Divergence of H2w and its progenies compared to reference strains in VP1/2A region (%)
| H2w | H2K5 | H2K10 | H2K15 | K2K20 | H2K25 | H2K30 | |
| HM175 | 10.1 | 3.6 | 3.6 | 3.6 | 3.6 | 3.6 | 3.6 |
| LA | 4.8 | 7.7 | 7.7 | 7.7 | 7.7 | 7.7 | 7.7 |
- Citation: Tang CH, Mao JS, Chai SA, Chen Y, Zhuang FC. Molecular evolution of hepatitis A virus in a human diploid cell line. World J Gastroenterol 2007; 13(34): 4630-4635
- URL: https://www.wjgnet.com/1007-9327/full/v13/i34/4630.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i34.4630