H pylori seropositivity and cytokine gene polymorphisms

Table 1 Characteristics of H pylori-seropositive and -seronegative subjects

	H pylori-seropositive(n = 237)		H pylori-seronegative(n = 173)		P-value
	n (%)		n (%)
Gender					0.645
Male	234	98.7	171	98.8
Female	3	1.3	2	1.2
Age (yr)					< 0.001
< 45	30	12.7	63	36.4
45-49	62	26.2	53	30.6
50-54	85	35.9	43	24.9
≥ 55	60	25.3	14	8.1
Smoking					0.281
Never	59	24.9	32	18.5
Former	76	32.1	57	32.9
Current	102	43.0	84	48.6
Drinking					0.023
Never or rarely	35	14.8	43	24.9
1-5 times/wk	131	55.3	91	52.6
6-7 times/wk	71	30.0	39	22.5

Table 2 H pylori seropositivity according to cytokine genotypes

	H pylori-seropositive(n = 237)		H pylori-seronegative(n = 173)		P-value
	n	(%)	n	(%)
IL1B-511C/T					0.243
CC	93	39.2	54	31.2
CT	109	46.0	89	51.4
TT	35	14.8	30	17.3
IL1B-31C/T					0.434
CC	33	13.9	29	16.8
CT	112	47.3	87	50.3
TT	92	38.8	57	32.9
IL6-634C/G					0.753
CC	138	58.2	104	60.1
CG	88	37.1	59	34.1
GG	11	4.6	10	5.8
TNF-1031T/C		0.0			0.170
TT	152	64.1	115	66.5
CT	80	33.8	51	29.5
CC	5	2.1	7	4.0
TNF-857C/T					0.018
CC	170	71.7	115	66.5
CT	64	27.0	47	27.2
TT	3	1.3	11	6.4
IL10-1082A/G					0.852
AA	211	89.0	153	88.4
AG/GG1	26	11.0	20	11.6

¹Only one subject had the IL10-1082 GG genotype.

Table 3 Age, gender-adjusted ORs for H pylori seropositivity according to cytokine genotypes

	n	Hp (+)%1	Adjusted OR (95%CI)	P-value
IL1B-511C/T
CC	147	63.3	1.00
CT	198	55.1	0.69 (0.43-1.10)	0.121
TT	65	53.8	0.70 (0.37-1.32)	0.270
IL1B-31C/T
CC	62	53.2	1.00
CT	199	56.3	1.11 (0.61-2.05)	0.726
TT	149	61.7	1.47 (0.78-2.78)	0.234
IL6-634C/G
CC	242	57.0	1.00
CG	147	59.9	1.06 (0.68-1.66)	0.785
GG	21	52.4	0.63 (0.24-1.62)	0.335
TNF-1031T/C
TT	267	56.9	1.00
CT	131	61.1	1.24 (0.78-1.95)	0.361
CC	12	41.7	0.48 (0.13-1.70)	0.253
TNF-857C/T
CC	285	59.6	1.00
CT	111	57.7	0.93 (0.58-1.49)	0.760
TT	14	21.4	0.15 (0.03-0.59)	0.007
IL10-1082A/G
AA	364	58.0	1.00
AG/GG2	46	56.5	1.08 (0.56-2.09)	0.811

¹H pylori seropositivity (%);

²Only one subject had the IL10-1082 GG genotype.

Table 4 Age, gender-adjusted ORs for H pylori seropositivity according to cytokine genotypes and lifestyle factors

	IL1B–511C/T
	n	Hp (+)%1	C/C	C/T	P-value	T/T	P-value
All subjects	410	57.8	1.00	0.69 (0.43-1.10)	0.121	0.70 (0.37-1.32)	0.270
Smoking
Never	91	64.8	1.00	0.30 (0.10-0.90)	0.032	0.40 (0.10-1.62)	0.200
Former	133	57.1	1.00	0.90 (0.39-2.10)	0.819	0.74 (0.26-2.15)	0.583
Current	186	54.8	1.00	0.83 (0.42-1.63)	0.582	0.80 (0.30-2.16)	0.658
Drinking
Never or rarely	78	44.9	1.00	0.58 (0.20-1.66)	0.310	1.38 (0.31-6.23)	0.676
1-5 times/wk	222	59.0	1.00	0.81 (0.43-1.55)	0.531	0.38 (0.16-0.95)	0.039
6-7 times/wk	110	64.5	1.00	0.66 (0.25-1.69)	0.383	1.11 (0.33-3.77)	0.862
	IL1B–31C/T
	n	Hp (+)%1	C/C	C/T	P-value	T/T	P-value
All subjects	410	57.8	1.00	1.11 (0.61-2.05)	0.726	1.47 (0.78-2.78)	0.234
Smoking
Never	91	64.8	1.00	0.79 (0.22-2.29)	0.723	2.25 (0.56-9.08)	0.257
Former	133	57.1	1.00	1.34 (0.50-3.62)	0.560	1.55 (0.55-4.42)	0.409
Current	186	54.8	1.00	1.20 (0.44-3.24)	0.722	1.22 (0.44-3.40)	0.705
Drinking
Never or rarely	78	44.9	1.00	0.44 (0.11-1.82)	0.258	0.68 (0.15-3.12)	0.624
1-5 times/wk	222	59.0	1.00	2.59 (1.04-6.47)	0.042	3.17 (1.23-8.14)	0.017
6-7 times/wk	110	64.5	1.00	0.72 (0.23-2.29)	0.579	0.81 (0.24-2.73)	0.735
	IL6–634C/G
	n	Hp (+)%1	C/C	C/G	P-value	G/G	P-value
All subjects	410	57.8	1.00	1.06 (0.68-1.66)	0.785	0.63 (0.24-1.62)	0.335
Smoking
Never	91	64.8	1.00	0.54 (0.22-1.38)	0.200	2.79 (0.28-27.73)	0.382
Former	133	57.1	1.00	0.63 (0.28-1.41)	0.259	0.00 (0.00)	0.999
Current	186	54.8	1.00	2.28 (1.13-4.58)	0.021	0.84 (0.22-3.15)	0.796
Drinking
Never or rarely	78	44.9	1.00	1.07 (0.41-2.82)	0.886	0.00 (0.00)	1.000
1-5 times/wk	222	59.0	1.00	1.02 (0.55-1.91)	0.940	0.40 (0.11-0.41)	0.153
6-7 times/wk	110	64.5	1.00	1.61 (0.63-4.12)	0.325	1.60 (0.28-9.28)	0.599

¹H pylori seropositivity (%).

Table 5 Age, gender-adjusted ORs for H pylori seropositivity according to the combination of the two promoter genotypes of TNF and IL1B

	n	Hp (+)%1	Adjusted OR (95%CI)	P-value
TNF-1031/-857
TT/CC2	169	58.6	1.07 (0.70-1.64)	0.743
TC/CC2	104	63.5	1.38 (0.85-2.25)	0.195
CC/CC2	12	41.7	0.44 (0.13-1.57)	0.207
TT/CT2	84	59.5	1.06 (0.63-1.78)	0.830
TC/CT2	27	51.9	0.89 (0.39-2.02)	0.781
TT/TT2	14	21.4	0.15 (0.04-0.60)	0.007
IL1B-511/-313
CC/TT2	141	61.7	1.29 (0.83-2.01)	0.259
CT/CT2	191	54.5	0.74 (0.48-1.13)	0.159
TT/CC2	59	52.5	0.77 (0.43-1.39)	0.384

¹H pylori seropositivity (%).

²Each reference group represented all the other combinations of genotypes.

³ORs of the groups with < 7 subjects were not analyzed: CT/TT = 4, TT/TT = 4, CC/CT = 6, TT/CC = 2, and CT/CC = 3.