Identification of the differential expressive tumor associated genes in rectal cancers by cDNA microarray

Table 1 Clinical data of the rectal cancer patients

Patient No.	Age (yr)	Sex	TNM	Clinical stage
1	31	Male	T4N2M0	III
2	51	Female	T3N1M0	III
3	47	Male	T3N1M0	III
4	67	Female	T3N0M0	II
6	49	Male	T2N2M0	III
7	38	Male	T3N0M0	II
8	59	Female	T3N0M0	II
9	53	Male	T3N0M0	II
10	62	Male	T2N0M0	I
11	62	Male	T3N1M0	III
12	41	Male	T3N1M0	III
13	33	Male	T4N0M0	II
14	76	Male	T3N1M0	III
15	75	Female	T2N0M0	I
16	32	Female	T3N1Mo	III
17	65	Female	T3N1Mo	II
18	48	Female	T3N0Mo	II
19	32	Male	T3N2M0	III
20	68	Male	T2N0M0	I
21	76	Male	T3N0M0	II
22	76	Male	T3N0M0	II

T1-4 represent the size of the tumor; N0-4 represent the level of node metastasis; M1-4 represent the level of metastasis.

Table 2 Commonly up-regulated genes in rectal cancer patients showing ≥ 2 fold over expression

AccessionNo.	Gene description	Patients(n)
NM_000184	Hs.23763, hemoglobin, gamma G (186624)	8
NM_000633	B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha (3950016)	6
Not found	Hs.77579, ESTs (2811005)	6
Not found	Hs.87497, ESTs (1216395)	5
BB366468	Hs.126701, ESTs, Weakly similar to homolog of the Aspergillus nidulans sudD gene product [H.sapiens] (1082187)	5
NM_000600	Interleukin 6 (interferon, beta 2) (IL6) (3876176)	5
NM_004469	c-fos induced growth factor (vascular endothelial growth factor D) (FIGF) (160946)	5
BM440387	KIAA0712 gene product (539641)	5
BB217505	Hs.106513, Homo sapiens mRNA; cDNA DKFZp586I1518 (from clone DKFZp586I1518) (585388)	5
NM_002210	Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) (ITGAV) (3878920)	5
L12168	Adenylyl cyclase-associated protein (2217802)	5
NM_007047	Hs.19301, butyrophilin, subfamily 3, member A2 (1070574)	5
NM_018844	b-cell receptor associated protein (2746778)	5
U41371	Spliceosome associated protein 145 (1964680)	5
NM_001022	Hs.5621, ribosomal protein S19 (728984)	5
NM_002506	Nerve growth factor, beta polypeptide (NGFB) (72869)	5
NM_000121	Erythropoietin receptor (EPOR) (114048)	5
NM_003371	Vav 2 oncogene (VAV2) (3861692)	5
NM_000267	Neurofibromin 1 (neurofibromatosis, von Recklinghausen disease, Watson disease) (NF1) (3868563)	5
AF071400	Plasminogen activator inhibitor, type II (arginine-serpin) (PAI2) (323255)	5
NM_006059	Laminin, gamma 3 (LAMC3) (2497685)	5
NM_002865	Hs.234726, RAB2, member RAS oncogene family (728947)	5
NM_013230	Hs.180414, CD24 antigen (small cell lung carcinoma cluster 4 antigen) (115306)	5

The number in the brackets represents IMAGE clone number.

Table 3 Commonly down-regulated genes in rectal cancers showing < 0.5 under expression

AccessionNo.	Gene description	Patients(n)
Not found	Hs.170311, EST (126182)	5
NM_004689	Metastasis associated 1 (MTA1) (4054392)	5
BC001766	S100 calcium-binding protein, beta (neural) (S100B) (759948)	5
NM_005246	Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) (FER) (3886018)	5
NM_001920	Decorin (DCN) (274397)	5
NM_001951	E2F transcription factor 5, p130-binding (E2F5) (701492)	5
NM_002211	Homo sapiens integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1) (531839)	5
Not found	Hs.23954, ESTs (132543)	5
Not found	Hs.5621, ESTs (2947053)	5
NM_007191	Wnt inhibitory factor-1 (WIF-1) (40908)	5
NM_021785	Retinoic acid induced 2 (RAI2) (501868)	5
Not found	Tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) (TAC1), transcript variant beta (784179)	5
NM_004345	Cathelicidin antimicrobial peptide (CAMP) (3057931)	5
AF385430	Rho GTPase activating protein 1 (ARHGAP1) (3896574)	5
NM_005252	v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) (755279)	5

The numbers in the brackets represent IMAGE clone number.