E-cadherin and beta-catenin expression in Epstein-Barr virus-associated gastric carcinoma and their prognostic significance

Table 1 Membranous and cytoplasmic expression of E-cadherin in EBV-GCs and non EBV-GCs

Location of E-cadherin expression	Number of the cases (%)		OR1	95% CI1
	EBV-GCs	non EBV-GCs
Total	59	120
Membranous
Preserved (> 50%)	14 (24)	29 (24)	1	Referent
Lost (< 50%)	45 (76)	91 (76)	0.99	0.48-2.05
Cytoplasm
Negative	26 (44)	59 (49)	1	Referent
Positive (subtotal)	33 (56)	61 (51)	1.28	0.65-2.50
10%-	17 (29)	29 (24)	1.37	0.62-3.01
50%-	11 (19)	29 (24)	0.92	0.39-2.18
90%-	5 (8)	3 (3)	3.98	0.88-18.0
			P = 0.374

¹ORs and 95% CIs were obtained from conditional logistic regression analysis with the objective variable being EBV-GC (EBV-GC = 1, and non EBV-GC = 0).

Table 2 Membranous and nuclear and/or cytoplasmic expression of beta-catenin in EBV-GCs and non EBV-GCs

Location ofbeta-catenin expression	Number of the cases (%)		OR1	95% CI1
	EBV-GCs	non EBV-GCs
Total	59	120
Membranous
Preserved (> 10%)	49 (83)	78 (65)	1	Referent
Lost (< 10%)	10 (17)	42 (35)	0.41	0.19-0.90
Nuclei and cytoplasm
Negative	46 (78)	106 (88)	1	Referent
Positive (subtotal)	13 (22)	14 (12)	2.23	0.97-5.09
10%-	5 (8)	8 (7)	1.48	0.47-4.66
50%-	6 (10)	6 (5)	2.34	0.74-7.36
90%-	2 (3)	0 (0)	-
			P = 0.024

¹ORs and 95% CIs were obtained from conditional logistic regression analysis with the objective variable being EBV-GC (EBV-GC = 1, and non EBV-GC = 0).

Table 3 Results of survival analysis in EBV-GC and non EBV-GC cases

	Non EBV-GCs			EBV GCs
	Death1/Total (%)	HR2	95% CI2	Death1/Total (%)	HR2	95% CI2
Depth
m, sm	6/29 (21)	1	Referent	3/13 (23)	1	Referent
pm	3/13 (23)	0.76	0.19-3.07	2/8 (25)	1.64	0.23-11.9
s, ss, se	37/58 (64)	2.03	0.74-5.53	18/28 (64)	6.03	1.39-26.2
		P = 0.112			P = 0.005
Lymph node metastasis
0	10/51 (20)	1	Referent	13/32 (41)	1	Referent
1	16/26 (62)	3.55	1.44-8.77	4/9 (44)	2.13	0.57-7.94
2+	20/23 (87)	6.83	2.75-17.0	6/8 (75)	7.77	1.93-31.3
		P < 0.001			P = 0.004
Lauren classification
Diffuse	14/30 (47)	1	Referent	6/20 (30)	1	Referent
Intestinal	32/70 (46)	1.10	0.55-2.02	17/29 (59)	9.47	2.67-33.6
		P = 0.865			P < 0.001
p53
Negative	21/52 (40)	1	Referent	13/26 (50)	1	Referent
Positive	25/48 (52)	1.60	0.87-2.91	10/23 (43)	1.29	0.50-3.30
		P = 0.127			P = 0.595
E-cadherin in membrane
Preserved	9/28 (32)	1	Referent	6/12 (50)	1	Referent
Lost	37/72 (51)	1.50	0.70-3.22	17/37 (46)	0.73	0.25-2.12
		P = 0.276			P = 0.568
E-cadherin in nuclei and/or cytoplasm
Negative	30/62 (48)	1	Referent	12/26 (46)	1	Referent
Positive	16/38 (42)	0.56	0.30-1.06	11/23 (48)	1.37	0.56-3.34
		P = 0.071			P = 0.481
Beta-catenin in membrane
Preserved	28/64 (44)	1	Referent	19/43 (44)	1	Referent
Lost	18/36 (50)	1.10	0.57-2.11	4/6 (67)	1.39	0.42-4.60
		P = 0.778			P = 0.601
Beta-catenin in nuclei and/or cytoplasm
Negative	41/89 (20)	1	Referent	18/37 (49)	1	Referent
Positive	5/11 (45)	0.81	0.31-2.09	5/12 (42)	0.32	0.11-0.93
		P = 0.654			P = 0.028

¹Gastric-cancer related death.

²HRs, 95% CIs, and P values were obtained by Cox proportional hazard models. Age, depth, lymph node metastasis, Lauren classification, and p53 expression were included in the models as covariates. The coefficient of age was 0.029 (SE = 0.018, P = 0.114) in non EBV-GCs, and 0.076 (SE = 0.026, P = 0.004) in EBV-GCs. m: mucosa, sm: submucosa, pm: propia muscularis, s: serosa, ss: subserosa, se: serosa exposed.