Overexpression of connective tissue growth factor WISP-1 in Chinese primary rectal cancer patients

Table 1 Primers and probes

	Sequence
Target gene/WISP-1
Forward primer	5’- CCACCGGGGCCTCTACT-3’
Reverse primer	5’- CCACACCGACCACCTGT-3’
Probes	5’-FAM-CTATTGCGTACCTCGGGCGGTC -TAMRA-3’
Reference gene/GAPDH
Forward primer	5’-CCTCAAGATCATCAGCAAT-3’
Reverse primer	5’-CCATCCACAGTCTTCTGGGT-3’
Probes	5’-FAM-ACCACAGTCCATGCCATCAC -TAMRA-3’

Table 2 Output of randomization test in two groups

	Reference gene (GAPDH)	Target gene (WISP-1)
Efficiency	1.61	1.85
Control group
n	86	86
Mean	25.43	38.91
Standard deviation	15.86	58.89
CV (%)	62.35	151.36
Sample group
n	86	86
Mean	24.74	35.04
Standard deviation	14.3	37.1
CV (%)	57.8	105.88

Table 3 Relationship between mRNA WISP-1 status and standard clinical, pathological factors

		WISP-1 mRNA expression
Factors	n	-	+	P
Age(yr)
< 60	48	16	32	0.7351
≥ 60	38	14	24
Sex
Female	42	11	31	0.0981
Male	44	19	25
Histological differentiation
Poor	18	2	16
Moderate	63	25	38	0.0392
Well	5	3	2
CEA3 (ng/mL)
≤ 3.4	20	9	11	0.1261
> 3.4	22	5	17
Lymph node status
Negative	51	24	27	0.0041
Positive	35	6	29
Duke’s staging4
A + B	47	22	25	0.0111
C + D	39	8	31
Histological type
Tubular adenocarcinoma	37	17	20	0.0811
Papillary adenocarcinoma	33	22	11

¹χ² test;

²Non-parametric test (Mann-Whitney);

³Tumor marker. Information is available for 42 patients;

⁴Duke’s staging A and B were combined, and C and D were combined.