Anti-tumor activities and apoptosis-regulated mechanisms of bufalin on the orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice

Table 1 Tumor volume, weight and tumor inhibitory rate pre- and post-treatment (mean ± SD, n = 10)

Group	Tumor		Tumor inhibitory	weight
	Volume (cm3)		rate	(g)
	Pre-treatment	Post- treatment	(%)	Pre-treatment	Post-treatment
BF1	43.50 ± 5.09	35.21 ± 12.51ac	79.3	20.94 ± 1.00	21.39 ± 1.62e
BF2	42.92 ± 4.10	49.83 ± 11.46ac	70.7	20.03 ± 1.16	21.48 ± 1.10e
BF3	43.00 ± 2.97	83.99 ± 24.63a	50.7	21.06 ± 0.94	21.57 ± 1.14e
ADM	42.93 ± 4.23	55.17 ± 16.13a	67.6	21.00 ± 1.00	18.90 ± 0.77
NS	43.37 ± 4.82	170.39 ± 25.29	-	20.95 ± 1.07	20.40 ± 1.23

^aP < 0.001 vs NS group;

^cP < 0.05 vs ADM group;

^eP < 0.05 vs ADM group.

Table 2 Mean survival and life prolonging rate of each group (mean ± SD)

Group	n	Mean survivaltime (d)	Life prolongingrate (%)
BF1	5	31.8 ± 4.2ac	35.9
BF2	5	29.4 ± 3.4ac	25.6
BF3	5	28.4 ± 3.9c	31.8
ADM	5	22.2 ± 1.6	21.4
NS	5	23.4 ± 2.1	-

^aP < 0.05 vs NS group;

^cP < 0.05 vs ADM group.

Table 3 The expression of bcl-2 or bax protein of tumor tissues in nude mice (mean ± SD, n = 10)

Group	bcl-2	Positiverate (% )	P	bax	Positiverate (%)	P
	- +			- +
BF1	9 1	10	> 0.05	1 9	90a	< 0.01
BF2	9 1	10	> 0.05	2 8	80c	< 0.05
BF3	8 2	20	> 0.05	2 8	80c	< 0.05
ADM	9 1	10	> 0.05	6 4	40	> 0.05
NS	8 2	20	-	7 3	30	-

^aP < 0.01 vs NS group;

^cP < 0.05 vs NS group.