Prognostic significance of HIF-2α/EPAS1 expression in hepatocellular carcinoma

Table 1 Expression of HIF-2α/EPAS1 in HCC

	Less thanparaneoplastictissue	Equal toparaneoplastictissue	Greater thanparaneoplastictissue
HIF2α/EPAS1	9 (4.8%)	59 (31.6%)	119 (63.6%)

Table 2 Correlation of clinicopathologic parameters with HIF-2α/ EPAS1 expression

		HIF-2α/EPAS1
Clinicopathologic parameters	n	Positive	Negative	χ2	P
Age (yr)
≥ 65	214	143	71	2.299	0.129
< 65	101	76	25
Gender
Male	260	175	85	0.452	0.063
Female	55	44	11
Liver cirrhosis
Present	142	101	41	0.314	0.576
Absent	173	118	55
Hepatitis B
Present	212	144	68	0.783	0.376
Absent	103	75	28
Hepatitis C
Present	22	17	5	0.67	0.413
Absent	293	202	91
Child’s classification
A	182	129	53	0.371	0.541
B	133	90	43
Tumor size (cm)
≤ 2	62	20	42
2-5	97	52	45	3.417	0.065
> 5	156	147	9
Tumor capsule
Present	118	87	31	1.575	0.21
Absent	197	132	65
Capsule infiltration
Present	75	61	14	6.479	0.011
Absent	240	158	82
Vascular invasion
Present	73	62	11	10.646	0.001
Absent	242	157	85
Edmondson-Steiner class
I	78	29	49
II	100	53	47	5.081	0.024
III	137	137	0
Intrahepatic metastasis
Present	97	75	22	4.02	0.045
Absent	218	144	74
Necrosis
Present	108	67	41	4.348	0.037
Absent	207	152	55

Table 3 Correlation of HIF-2α/EPAS1 expression with VEGF expression and MVD in HCC

		HIF-2α/EPAS1 expression
Parameters	n	Positive	Negative	P
VEGF
Weak	56	33	23
Moderate	70	40	30
Strong	114	82	32	0
Negative	75	64	11
MVD
Low	99	57	42
Medium	103	71	32	0.016
High	113	91	22

Table 4 Multivariate survival analysis (Cox’s regression)

Parameters	β	SE	Relative risk	P	95% CI
Tumor size (cm) ( ≤ 2-5 vs≥ 5)	0.341	0.183	1.659	0.766	0.762-0.456
Edmondson-Steiner class (I, II vs III)	0.157	0.095	1.17	0.91	0.986-1.083
Necrosis (no vs yes)	0.252	0.158	1.287	0.881	0.425-5.820
Venous invasion (no vs yes)	0.079	0.13	1.521	0.001	2.564-17.617
Tumor capsule (no vs yes)	0.366	0.149	1.269	0.633	0.824-1.544
Capsule infiltration (no vs yes)	0.552	0.148	1.737	0.832	0.562-5.234
Intrahepatic metastasis (no vs yes)	0.477	0.153	1.611	0.002	1.081-3.872
Child’s class (A vs B)	0.592	0.173	1.646	0.373	0.195-1.873
HIF-2α/EPAS1 (no vs yes)	1.308	0.176	3.699	0	1.385-10.236