Effect of targeted magnetic nanoparticles containing 5-FU on expression of bcl-2, bax and caspase 3 in nude mice with transplanted human liver cancer

Table 1 Comparison of gross tumor volume (mm³, mean ± SD)

Group	n	0	1d	4d	7d	10d	13d
A	8	131.9 ± 4.3	261.0 ± 29.7	362.0 ± 52.9	513.1 ± 67.3	643.9 ± 83.4	776.1 ± 116.2
B	8	133.7 ± 8.3	253.0 ± 27.8	341.5 ± 34.6	473.4 ± 53.2	600.2 ± 72.4	764.7 ± 100.6
Ca	8	134.4 ± 4.7	228.7 ± 26.7	294.8 ± 31.6	364.9 ± 34.5	416.9 ± 64.4	465.0 ± 91.1
Dab	8	135.8 ± 6.8	213.0 ± 18.1	261.5 ± 22.0	315.5 ± 27.6	337.2 ± 32.6	367.8 ± 44.0

^aP < 0.01 vs groups A and B;

^bP < 0.05 vs group C.

Table 2 Intensity of staining of bcl-2, bax and caspase 3 proteins (mean ± SD)

Group	n	Bcl-2	Bax	Caspase 3
A	8	0.1185 ± 0.0111	0.1031 ± 0.0119	0.0806 ± 0.0073
B	8	0.1161 ± 0.0094	0.1090 ± 0.0141	0.1067 ± 0.0075
C	8	0.0981 ± 0.0121a	0.1375 ± 0.0084a	0.1284 ± 0.0074a
D	8	0.0768 ± 0.0119ab	0.1587 ± 0.0086ab	0.1468 ± 0.0080ab

^aP < 0.01 vs groups A and B;

^bP < 0.01 vs group C.

Table 3 mRNA expression in bcl-2, bax and caspase 3 (mean ± SD)

Group	Bcl-2	Bax	Caspase 3
A	0.557 ± 0.064	0.136 ± 0.012	0.134 ± 0.012
B	0.524 ± 0.049	0.187 ± 0.019	0.226 ± 0.028
C	0.275 ± 0.025a	0.681 ± 0.057a	0.425 ± 0.036a
D	0.154 ± 0.014ab	0.821 ± 0.065ab	0.557 ± 0.039ab

^aP < 0.01 vs group A;

^bP < 0.01 vs group C.

Table 4 Expression of bcl-2, bax and caspase 3 proteins (mean ± SD)

Group	Bcl-2	Bax	Caspase3
A	0.415 ± 0.052	0.086 ± 0.012	0.062 ± 0.006
B	0.402 ± 0.048	0.182 ± 0.028	0.145 ± 0.017
C	0.216 ± 0.033a	0.382 ± 0.044a	0.409 ± 0.024a
D	0.156 ± 0.018ab	0.508 ± 0.058ab	0.628 ± 0.033ab

^aP < 0.01 vs group A;

^bP < 0.01 vs group C.