Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats

Table 1 Effects of 5-FU and 5-FU + G-CSF administration on peritoneal defense mechanisms and systemic WBC counts. Figures are expressed as median (range)

	Control (n: 10)	5-FU (n: 10)	5-FU + G-CSF (n: 10)
Bactericidal activity of peritoneal fluid MIC90 (bacteria/mL)	17.90 (14.1-24.6)	9.40 (6.2-13.8)a	19.20 (14.3-26.8)
Phagocytic activity of PMNL	56.3 (48.3-62.6)	36 (25-51.6)a	43.5 (37-58.6)
Cell counts in peritoneal fluid	2000 (1500-2400)	950 (600-1200)a	1380 (800-1900)
Systemic WBC Counts	9000 (4000-14000)	2100 (1100-2600)a	3400 (2200-4600)

^aP < 0.05 vs control and 5-FU + G-CSF groups.

Table 2 Cell types in peritoneal fluid. Values are presented as median (range)

Groups	n	Neutrophil/mm3	Macrophage/mm3
Control	10	88 (80-94)	14 (1-20)
5-FU	10	68 (62-92)a	8 (6-12)a
5-FU + G-CSF	10	80 (73-87)	12 (6-15)

^aP < 0.05 vs control and 5-FU + G-CSF groups.

Table 3 Bacterial translocation of the groups

	Control	5-FU	5-FU + G-CSF
Spleen	0	0	0
Liver	0	2	0
Mesenteric lymph node	1	5	0