Gastric Cancer
Copyright ©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. May 14, 2007; 13(18): 2568-2574
Published online May 14, 2007. doi: 10.3748/wjg.v13.i18.2568
Table 1 Primer sequences (5'-3') for MSP
GeneSenseAntisenseProduct sizeTM
CDH1M-ATTCGAATTTAGTGGAATTAGAATCM-CCCAAAACGAAACTAACGAC125 bp53°C
U-GGATTTGAATTTAGTGGAATTAGAATTU-CTCCCCAAAACAAAACTAACAAC130 bp
FHITM-TTTTCGTTTTTGTTTTTAGATAAGCM-AAAAATATACCCACTAAATAACCGC157 bp52°C
U-TGGTTTTTGTTTTTGTTTTTAGATAAGTU-AAAATATACCCACTAAATAACCACC159 bp
MTAPM-TGTTTTTTAGGAATTAAGGGAAATACM-AACTACAAAATCTAACCCGACGAC199 bp52°C
U-TTTTTAGGAATTAAGGGAAATATGTU-CAACTACAAAATCTAACCCAACAAC197 bp
PLAGL1M-GTTTATTTTGGCGGAGATTTCM-ACTAAACGACACCCACACGTC147 bp51°C
U-GGTTTATTTTGGTGGAGATTTTGU-AAAAACTAAACAACACCCACACAT152 bp
Table 2 Clinico-pathological characteristics of the samples
Variable (n)SamplesP
Nonneoplastic n (%)Intestinal n (%)Diffuse n (%)
Gender
Male (54)9 (16.67)20 (37.03)25 (46.3)0.9176
Female (24)4 (16.66)10 (41.67)10 (41.67)
Age (yr)
≤ 50 (23)7 (30.43)7 (30.43)9 (39.14)0.1056
> 50 (55)6 (10.91)23 (41.82)26 (47.27)
Smoking status
Smoker (27)1 (3.7)13 (48.15)13 (48.15)0.0717
No smoker (51)12 (23.53)17 (33.33)22 (43.14)
H pylori
Present (36)6 (16.67)8 (22.22)22 (61.11)0.0142a
Absent (42)7 (16.67)22 (52.38)13 (30.95)
Location
Cardia (25)-11 (44)14 (56)0.783
Noncardia (40)-19 (47.5)21 (52.5)
T stage
T1, T2 (15)-7 (46.67)8 (53.33)0.9638
T3, T4 (50)-23 (46)27 (54)
Lymph node metastasis
Present (51)-23 (45.1)28 (54.9)0.7445
Absent (14)-7 (50)7 (50)
Distant metastasis
Present (6)-4 (66.67)2 (33.33)0.4649
Absent (55)-23 (41.82)32 (58.18)
Unknown (4)-3 (75%)1 (25%)
Table 3 Methylation number and frequency in gastric tissue samples, n (%)
SamplesMethylation status
CDH1
FHIT
MTAP
PLAGL1
M/UUM/UUM/UMPM/UM
Normal tissue (13)12 (92.31)1 (7.69)8 (61.54)5 (38.46)11 (84.62)2 (15.38)12 (92.31)1 (7.69)
GC (65)65 (100)0 (0)34 (52.31)31 (47.69)49 (75.38)16 (24.62)43 (66.15)22(33.85)
Diffuse-type GC (35)35 (100)0 (0)17 (48.57)18 (56.25)22 (62.86)13 (37.14)0.0249a23 (65.71)12(34.29)
Intestinal-type GC (30)30 (100)0 (0)17 (56.67)13 (43.33)27 (90)3 (10)20 (66.6)10(33.33)
Table 4 Number of hypermethylated genes in gastric tissue samples
Samples (n)Number of hypermethylated genes
P value
1 or 23 or 4
Nonneplastic gastric tissue (13)12 (92.31%)1 (7.69%)
Gastric cancer (65)44 (67.69%)21 (32.31%)0.0396b
Diffuse-type GC (35)21 (60%)14 (40%)
Intestinal-type GC (30)23 (76.67%)7 (23.33%)