Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker

Table 1 List of primer sequences used in the study

Gene ID	Primer sequence	Productsize
AFP	Sense TGCAGCCAAAGTGAAGAGGGAAGA	216 bp
	Antisense CATAGCGAGCAGCCCAAAGAAAGAAA
Albumin	Sense TGCTTGAATGTGCTGATGACAGGG	161 bp
	Antisense AAGGCAAGTCAGCAGGCATCTCATC
CK-18	Sense ATGGGCGAGCAGAACCGGAA	326 bp
	Antisense CCATGAGCCGCTGGTACTCC

Table 2 Enrichment of Hepatic progenitor cells using anti-CD34 by MACS

Gestation(wk)	Total cell yield	% of CD34+/CD45+	% of CD34+/CD45-
13-15	39 + 2.3 × 106	5.5 + 0.56	4.1 + 0.82
16-18	65 + 2.9 × 106	4.8 + 0.72	4.2 + 0.9
19-20	90 + 3.4 × 106	4.5 + 0.82	3.9 + 0.8

Each data was a representative of three replicates. The numbers of samples within individuals are six and three replicates in each set were done.

Table 3 AFP activities in anti-CD34 sorted cells

Group	AFP before sorting(ng/mg protein)	AFP after sorting(ng/mg protein)	Folds increase
I	835 + 16	4336 + 12	5.2 + 1.2
II	554 + 15	2557 + 14	4.9 + 1.3
III	384 + 13	1572 + 15	4.5 + 1.2

Each data was a representative of three replicates. The number of samples within individuals are six and three replicates in each set were done.