Rapid Communication
Copyright ©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 21, 2007; 13(15): 2209-2213
Published online Apr 21, 2007. doi: 10.3748/wjg.v13.i15.2209
Table 1 Primer sequence, PCR program and Genbank accession number
PrimersSense (5’ -> 3’)Antisense (5’ -> 3’)Size and PCR conditionGenBank accession number
β-actinGGCATCGCTGGA94°C 5 min; 92°C 40 s;BC023204
GTGATGAGGTGG58°C 40 s; 72°C 50 s, 30X
GAC TCC GACA GCG A
p21WAF1CAGGGGGGGCGG94°C 5 min; 94°C 1 min;NM_000389
ACAGCACCAGGG58°C 1 min; 72°C 1 min, 35X
GAG GAA GATAT GTA C
Table 2 Sequences of primers and probes for real-time PCR
GenePrimer (forward) (5’ -> 3’)Primer (reverse) (5’ -> 3’)ProbeGenBank No.
p21WAF1CTG GAG ACTGGA TTA GGGACG GCG GCANM_078467
CTC AGG GTCCTT CCT CTTGAC CAG CAT GA
GAAGGA
β-actinCTG GCA CCCGGA CAG CGAATC ATT GCTBC016045
AGC ACA ATGGGC CAG GATCCT CCT GAG
Table 3 Expression of p21WAF1 mRNA in colorectal carcinoma tissue
CanceroustissuesPara-cancerousNormal mucosa
p21WAF1 mRNA2377.95 ± 865.80b3216.58 ± 1149.423541.61 ± 433.17