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©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 21, 2007; 13(15): 2209-2213
Published online Apr 21, 2007. doi: 10.3748/wjg.v13.i15.2209
Published online Apr 21, 2007. doi: 10.3748/wjg.v13.i15.2209
Primers | Sense (5’ -> 3’) | Antisense (5’ -> 3’) | Size and PCR condition | GenBank accession number | ||
β-actin | GGC | ATC | GCT | GGA | 94°C 5 min; 92°C 40 s; | BC023204 |
GTG | ATG | AGG | TGG | 58°C 40 s; 72°C 50 s, 30X | ||
GAC TCC G | ACA GCG A | |||||
p21WAF1 | CAG | GGG | GGG | CGG | 94°C 5 min; 94°C 1 min; | NM_000389 |
ACA | GCA | CCA | GGG | 58°C 1 min; 72°C 1 min, 35X | ||
GAG GAA GA | TAT GTA C |
Gene | Primer (forward) (5’ -> 3’) | Primer (reverse) (5’ -> 3’) | Probe | GenBank No. |
p21WAF1 | CTG GAG ACT | GGA TTA GGG | ACG GCG GCA | NM_078467 |
CTC AGG GTC | CTT CCT CTT | GAC CAG CAT GA | ||
GAA | GGA | |||
β-actin | CTG GCA CCC | GGA CAG CGA | ATC ATT GCT | BC016045 |
AGC ACA ATG | GGC CAG GAT | CCT CCT GAG |
Canceroustissues | Para-cancerous | Normal mucosa | |
p21WAF1 mRNA | 2377.95 ± 865.80b | 3216.58 ± 1149.42 | 3541.61 ± 433.17 |
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Citation: Chen YX, Fang JY, Lu R, Qiu DK. Expression of
p 21WAF1 is related to acetylation of histone H3 in total chromatin in human colorectal cancer. World J Gastroenterol 2007; 13(15): 2209-2213 - URL: https://www.wjgnet.com/1007-9327/full/v13/i15/2209.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i15.2209