In vitro study of lovastatin interactions with amiodarone and with carbon tetrachloride in isolated rat hepatocytes

Table 1 Cell viability, LDH activity and concentrations of TBARS and GSH after 1 h incubation with lovastatin (mean ± SD)

Groups	Cell viability %	LDH nmol/min/106 cells	TBARSnmol/106 cells	GSH nmol/106 cells
Control	75 ± 5.97	0.157 ± 0.096	0.102 ± 0.018	13.0 ± 2.53
1 μmol/L Lovastatin	68 ± 0.051b	0.214 ± 0.084	0.138 ± 0.061	9.63 ± 1.19
3 μmol/L Lovastatin	52 ± 4.28d	0.248 ± 0.063b	0.158 ± 0.016b	4.68 ± 1.06b
5 μmol/L Lovastatin	48 ± 9.19d	0.272 ± 0.057b	0.169 ± 0.067b	3.93 ± 0.51b
10 μmol/L Lovastatin	41 ± 2.36d	0.336 ± 0.007b	0.194 ± 0.022d	2.05 ± 0.042d

^bP < 0.01 vs control;

^dP < 0.001 vs control.

Table 2 Cell viability, LDH activity and concentrations of TBARS and GSH after 1 h incubation with different concentrations lovastatin and 86 μmol/L CCl₄ (mean ± SD)

Group	Cell viability(%)	LDH nmol/min/106 cells	TBARS nmol/106 cells	GSHnmol/106 cells
Control	75 ± 5.97	0.157 ± 0.096	0.102 ± 0.018	13.0 ± 2.53
CCl4	35 ± 6.59f	0.563 ± 0.098f	0.304 ± 0.056f	1.24 ± 0.43f
1 μmol/L Lovastatin + CCl4	33 ± 4.99f	0.342 ± 0.028da	0.193 ± 0.004da	1.40 ± 0.49f
3 μmol/L Lovastatin + CCl4	42 ± 4.50d	0.328 ± 0.041a	0.170 ± 0.020a	2.49 ± 0.10f
5 μmol/L Lovastatin + CCl4	43 ± 7.77d	0.291 ± 0.025a	0.160 ± 0.014a	6.55 ± 0.005fb
10 μmol/L Lovastatin + CCl4	45 ± 1.52d	0.239 ± 0.016a	0.130 ± 0.041a	7.50 ± 0.008db

^aP < 0.05 vs CCl₄ group;

^bP < 0.01 vs CCl₄ group;

^dP < 0.01 vs control;

^fP < 0.001 vs control.

Table 3 Cell viability, LDH activity and concentrations of TBARS and GSH after 1 h incubation with 14 μmol/L amiodarone, 10 μmol/L lovastatin and 86 μmol/L CCl₄ (mean ± SD)

Group	Cellviability%	LDHnmol/min/106cells	TBARSnmol/106cells	GSHnmol/106cells
Control	75 ± 5.97	0.157 ± 0.096	0.102 ± 0.018	13.0 ± 2.53
86 μmol/L CCl4	35 ± 6.59j	0.563 ± 0.098j	0.304 ± 0.056j	1.24 ± 0.43j
10 μmol/L Lovastatin	41 ± 2.36j	0.336 ± 0.007f	0.194 ± 0.022j	2.05 ± 0.042h
10 μmol/L Lovastatin + 86 μmol/L CCl4	45 ± 1.52bj	0.239 ± 0.016a	0.130 ± 0.041af	7.50 ± 0.008d
14 μmol/L amiodarone	65 ± 2.71dj	0.229 ± 0.046b	0.156 ± 0.072ah	5.51 ± 1.06fj
14 μmol/L amiodarone + 86 μmol/L CCl4	39 ± 6.63j	0.270 ± 0.032af	0.162 ± 0.059ah	4.80 ± 0.284dj
14 μmol/L amiodarone + 10 μmol/L lovastatin	52 ± 4.58dfj	0.294 ± 0.032hl	0.166 ± 0.063ah	5.42 ± 1.04bjl
14 μmol/L amiodarone + 10 μmol/L lovastatin + 86 μmol/L CCl4	35 ± 2.22j	0.286 ± 0.052f	0.151 ± 0.077af	2.94 ± 1.61j

^aP < 0.05 vs CCl₄ group;

^bP < 0.01 vs CCl₄ group;

^dP < 0.001 vs CCl₄ group;

^fP < 0.01 vs control;

^hP < 0.01 vs control;

^jP < 0.01 vs control;

^lP < 0.01 vs lovastatin group.