Epithelial cells with hepatobiliary phenotype: Is it another stem cell candidate for healthy adult human liver?

Table 1 Medical history of donors

Age (yr)	Sex	Cause of death
4	Male	Cranial trauma
9	Male	Cranial trauma
16	Female	Trauma
18	Male	Trauma
19	Male	Cranial trauma
36	Male	Cardiac arrest
43	Male	Euthanasia
43	Female	Cranial trauma + Cerebral hemorrhage
43	Female	Cerebral hemorrhage
44	Female	Cerebral hemorrhage
47	Female	Pulmonary embolism
51	Male	Suicide
61	Male	Cerebral hemorrhage

Table 2 Primers used for RT- PCR analysis of LECs

Gene name	Primers	PCR productsize
AFP	5’-TGAAATGACTCCAGTAAACCC-3’	199 pb
	5’-GATGAAGCAAGAGTTTCTCATT-3’
Oct-4	5’-CGA CCA TCT GCC GCT TTG AG-3’	573 pb
	5’-CCC CCT GTC CCC CAT TCC TA-3’
Human hepatocyte nuclear factor 4 (HNF4)	5’-CCA AGT ACA TCC CAG CTT TC-3’	295 pb
	5’-TTG GCA TCT GGG TCA AAG-3’
ALB	5’-CCTTGGTGTTGATTGCCTTTGCTC-3’	308 pb
	5’-CATCACATCAACCTCTGGTCTCACC-3’
CK-8	5’-AAG GGC TGA CCG ACGAGA TC-3’	537 pb
	5’-GCT TCC TGT AGG TGG CGA TC-3’
Glucose 6 phosphatase (G6P)	5’-TCA GCT CAG GTG GTC CTC TT-3’	291 pb
	5’-CCT CCT TAG GCA GCC TTC TT-3’
α-antitrypsin (AAT)	5’-TCGCTACAGCCTTTGCAATG-3’	142 pb
	5’-GGAACTCCTCCGTACCCTCAA-3’
Tyrosine aminotran- sferase (TAT)	5’-TGA GCA GTC TGT CCA CTG CCT-3’	359 pb
	5’-ATG TGA ATG AGG AGG ATC TGA G-3’
Cytochrome P450 1B1 (CYP1B1)	5’-GAGAACGTACCGGCCACTATCACT-3’	357 pb
	5’-GTTAGGCCACTTCACTGGGTCATGAT-3’
CYP2B6	5’-CCT CTT CCA GTC CAT TAC CG-3’	551 pb
	5’-TGA CTG CCT CTG TGT ATG GC-3’
CYP3A4	5’-TGC TGT CTC CAA CCT TCA CC-3’	802 pb
	5’-TAG CTT GGA ATC ATC ACC ACC-3’
CK-19	5’-TTTGAGACGGAACAGGCTCT-3’	426 pb
	5’-CAGCTCAATCTCAAGACCCTG-3’
GGT	5’-GAC GAC TTC AGC TCT CCC AG-3’	489 pb
	5’-CTT GTC CCT GGA TTG CTT GT-3’
Multidrug resistance- associated protein 2 (MRP2)	5’-ACA CCA ACC AGA AAT GTG TC-3’	660 pb
	5’-CCA AGG CCT TCC AAA TCT C-3’
Glyceraldehyde- 3-phosphate dehydrogenase (GAPDH)	5’-CGGAGTCAACGGATTTGGTCGTAT-3’	307 pb
	5’-AGCCTTCTCCATGGTGGTGAAGAC-3’