Functional evaluation of a new bioartificial liver system in vivo and in vivo

Table 1 Changes of ALT, TBi and ALB in hepatocyte suspensions and RPMI-1640 media during circulation (mean±SD)

Portion	Time (h)	Biochemical parameters
		ALT(U/L)	TB(µmol/L)	ALB(g/L)
Hepatocyte suspensions (n = 5)	0	13.7 ± 3.5	3.5 ± 2.4	2.8 ± 1.5
	2	15.2 ± 4.5	5.0 ± 1.6c	3.1 ± 1.6
	4	17.9 ± 4.3	6.2 ± 1.8c	3.6 ± 0.9c
	6	18.4 ± 3.2	8.4 ± 2.6c	3.8 ± 1.2c
RPMI1640 media (n = 5)	0	0.8 ± 0.2a	0.0 ± 0.0c	0.5 ± 0.1a
	2	1.5 ± 0.5a	4.8 ± 1.3c	1.8 ± 0.3ac
	4	1.3 ± 0.3a	5.7 ± 1.6c	2.5 ± 0.2ac
	6	1.4 ± 1.0a	6.8 ± 2.5c	2.8 ± 0.3ac

^aP < 0.05 vs hepatocyte suspensions at the same circulation time,

^cP < 0.05 vs 0 h.

Table 2 Changes of biochemical parameters before and after circulation in two groups (mean±SD)

Group	Biochemical parameters
	ALT(U/L)	TB(μmol/L)	NH3(μmol/L)
BAL group (n = 8)
Pre-model	18.2 ± 12.6	2.8 ± 1.2	1.0 ± 0.0
Pre-circulation	325.8 ± 54.7c	72.6 ± 23.5c	132.2 ± 28.3c
Post-circulation	212.3 ± 42.3ac	33.4 ± 22.2ac	33.2 ± 21.7ac
Control group (n = 8)
Pre-model	16.6 ± 38.3	2.6 ± 1.9	1.0 ± 0.0
Pre-circulation	300.2 ± 52.6c	74.3 ± 23.7c	126.3 ± 30.4c
Post-circulation	280.1 ± 45.9c	65.5 ± 35.6c	100.3 ± 13.5c

^aP < 0.05 vs pre-circulation, ^cP < 0.05 vs pre-model.