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©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 28, 2006; 12(8): 1308-1311
Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1308
Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1308
Primers or probe | Sequence |
Reverse primers | |
Reaction V | 5’-CCCCCAATACCACATCATCC-3’ |
Reaction I | 5’-CCCCCAATACCACATCATCA-3’ |
Reaction C | 5’-CCCCCAATACCACATCATC-3’ |
Forward primers | 5’-tgaggagcacgagacggaagtATACAA CACCTGTATTCCCATCCCAT-3’ |
Probe | 5’-FAM ACTTCCGTCTCGTGCTCCTCA TAMRA-3’ |
Samples | HBV DNA(Copies/mL) | Result of UT-PCR | Result of sequencing |
1 | 2.49 × 105 | YMDD | 5′ -TATATGGATGAT-3′ |
2 | 4.73 × 107 | YIDD | 5′ -TATATTGATGAT-3′ |
3 | 6.10 × 103 | YMDD | 5′ -TATATGGATGAT-3′ |
4 | 6.24 × 106 | YIDD | 5′ -TATATTGATGAT-3′ |
5 | 2.98 × 109 | YIDD | 5′ -TATATTGATGAT-3′ |
6 | 8.13 × 106 | YMDD | 5′ -TATATGGATGAT-3′ |
7 | 1.35 × 104 | YMDD | 5′ -TATATGGATGAT-3′ |
8 | 3.57 × 103 | YMDD | 5′ -TATATGGATGAT-3′ |
9 | 2.74 × 106 | YVDD | 5′ -TATGTGGATGAT-3′ |
10 | 9.10 × 104 | YVDD | 5′ -TATGTGGATGAT-3′ |
11 | 7.02 × 104 | YMDD | 5′ -TATATGGATGAT-3′ |
12 | 5.18 × 105 | YMDD | 5′ -TATATGGATGAT-3′ |
13 | 3.51 × 103 | YMDD | 5′ -TATATGGATGAT-3′ |
14 | 8.04 × 104 | YMDD | 5′ -TATATGGATGAT-3′ |
15 | 4.29 × 106 | YMDD | 5′ -TATATGGATGAT-3′ |
- Citation: Wang RS, Zhang H, Zhu YF, Han B, Yang ZJ. Detection of YMDD mutants using universal template real-time PCR. World J Gastroenterol 2006; 12(8): 1308-1311
- URL: https://www.wjgnet.com/1007-9327/full/v12/i8/1308.htm
- DOI: https://dx.doi.org/10.3748/wjg.v12.i8.1308