Relationships between mucinous gastric carcinoma, MUC2 expression and survival

Table 1 Patients and sample characteristics

	UCPouch before closure of ileostomy	UCPouch in use, no pouchitis	UCpouchitis	FAPpouch before closure of ileostomy	FAPpouch in use, no pouchitis	FAPpouchitis	Healthy control	UC control term.ileurn without inflammation
Patients (n)	10	15	11	6	14	7	23	35
Age(yr)	17-36	19-45	27-50	17-27	19-45	22-36	17-53	18-59
Sex(f/m)	5/5	8/7	4/7	4/2	10/4	4/3	12/11	23/12
Pouch Classification:
Moskowitz	2-4	<4	>4	2-4	<4	>4	-	-

PDAI: pouchitis disease activity index (13); Values in points: <7: no pouchitis; >7: pouchitis Moskowitz: histologic classification of inflammatory changes in pouches (14). Values in points: <4 no pouchitis; >4: pouchitis UC: ulcerative colitis

Table 2 Nucleotide sequences of PCR primer (source: human)

Primer	Orientation	Sequence	PCR product size(bp)	GeneBank accession
hBD-1	Sense	5`-TTG TCT GAG ATG GCC TCA GGT GGT AAC	253	NM005218
	Antisense	5`-ATA CTT CAA AAG CAA TTT TCC TTT AT
hBD-2	Sense	5`-ATC AGC CAT GAG GGT CTT GT	173	AF448141
	Antisense	5`-GAG ACC ACA GGT GCC AAT TT
hBD-3	Sense	5`-AGC CTA GCA GCT ATG AGG ATC	206	NM018661
	Antisense	5`-CTT CGG CAG CAT TTT CGG CCA
HD5	Sens	5`-GCC ATC CCT GCT GCC ATT C	241	NM 021010
	Antisense	5`-AGA TTT CAC ACC CCG GAG A
HD6	Sense	5`-CCT CAC CAT CCT CAC TGC TGT TC	266	NM001926
	Antisense	5`-CCA TGA CAG TGC AGG TCC CAT A
TNFα	Sense	5`-TCA GCT TGA GGG TTT GCT ACA A	100	NM000594
	Antisense	5`-TCT GGC CCA GGC AGT CAG ATC
IL-1β	Sense	5`-CCA GCT ACG AAT CTC CGA CCA CCA CTA C	600	NM000576
	Antisense	5`-TGC TTG AGA GGT GCT GAT GTA CCA GTT G
IL-4	Sense	5`-AAC ACA ACT GAG AAG GAA ACC TTC T	276	NM000589
	Antisense	5`-GCT CGA ACA CTT TGA ATA TTT CTC
IL-6	Sense	5`-AGG AGC CCA GCT ATG AAC TCC TTC	120	NM000600
	Antisense	5`-TGG AAT CTT CTC CTG GGG GTA CTG
IL-8	Sense	5`-ATG ACT TCC AAG CTG GCC GTG GC	307	NM000584
	Antisense	5`-TCT CAG CCC TCT TCA AAA ACT TC

Table 3 Cytokine expression in ulcerative colitis or FAP pouches

Cytokine	Controls			Pouches
	Ileum control	Ileum in UC	UC ileostoma	UC without pouchitis	UC pouchitis	FAP ileostoma	FAP without pouchitis	FAP pouchitis
TNFα	+	(+)	+++	++	++	+	+	+
IL-1β	-	++	+++	+++	+++	+++	++	+
IL-4	-	-	(+)	(+)	(+)	+	(+)	(+)
Il-6	-	-	+	+	(+)	(+)	(+)	(+)
IL-8	(+)	++	++	++	++	++	+	(+)

UC: ulcerative colitis; FAP familial adenomatous polyposis cancer syndom. Samples were analysed by rt-PCR. +++: strong expression in all samples ++: medium expression in all samples +: weak expression in most samples (+): weak expression in some samples