Construction of retroviral vector of p125FAK specific ribozyme genes and its effects on BGC-823 cells

Table 1 Primer sequence and fragment length

Primer	Sequence	Fragment length (bp)	Annealin Temp (°C)
p125FAK	A: 5’TTCTTCTATCAACAGGTGAAG3’	632	55
	B: 5’CTGCGAGGTTCCATTCACCAG3’
β-actin ’	A: 5’GGCATGGGTCAGAAGGATTCC3	500	55
	B: 5’ATGTCACGCACGATTTCCCGC3’

Note: A and B represent the sense and anti-sense primers, respectively.

Table 2 Effect of RZ on colony formation of BGC-823 cells

Groups	CFE (%)	CIE (%)
Control group	51.24 ± 6.8a
Liposome group	46.43 ± 8.6a	9.38
Keno-vector group	45.32 ± 5.2a	11.55
Ribozyme group	16.14 ± 3.5a	68.5

^aP < 0.05 vs other groups.