Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray

Table 1 Characteristics of the studied patients (n = 58)

Characteristics	n (%)
Age (yr)
Range	48-68
mean ± SD	58.7 ± 3.46
Median	59
Mode	59
Gender
Male	30 (52)
Female	28 (48)
Tumor location
Gastric antrum	38 (66)
Gastric body	10 (17)
Gastric cardia	10 (17)
Anemia
Yes	48 (83)
No	10 (17)
Abdominal pain
Yes	27 (47)
No	31 (53)
Weight los
Yes	34 (59)
No	24 (41)
Occult blood test
Positive	43 (74)
Negative	25 (26)

Table 2 Differentially expressed genes detected by cDNA microarray

GeneBank	Description	Cy3:Cy52
NM_006500	MCAM (melanoma adhesion molecule)	10.791
NM_002545	OPCML (opioid binding protein/cell adhesion molecule-like)	10.561
NM_002933	RNASE1 (ribonuclease, RNase A family, 1)	6.81
NM_001993	F3 (coagulation factor III)	6.29
NM_005433	YES1(v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1	5.9161
NM_016525	UBAP (ubiquitin associated protein)	5.876
NM_001428	ENO1 (enolase 1)	5.6921
S68616	SLC9A1 (Na+/H+ exchanger NHE-1 isoform)	5.484
NM_003254	TIMP1 (tissue inhibitor of metalloproteinase 1)	5.291
NM_005903	MADH5 (mothers against decapentaplegic, homolog 5)	5.083
U82828	ATM (ataxia telangiectasia)	0.2051
NM_006343	MERTK (c-mer proto-oncogene tyrosine kinase)	0.19
NM_002985	SCYA5 (small inducible cytokineA5)	0.191
NM_005348	HSPCA (heat shock 90kD protein 1, alpha)	0.164
NM_003968	UBE1C (ubiquitin-activating enzyme E1C)	0.161
NM_004374	COX6C (cytochrome c oxidase subunit VIc)	0.131
NM_002990	SCYA22 (small inducible cytokine subfamily A, member22)	0.129
NM_005781	ACK1 (activated p21cdc42Hs kinase)	0.1281
NM_005139	ANXA3 (annexin A3)	0.124
AF053630	SERPINB1(serine proteinase inhibitor, clade B, member 1)	0.123
NM_012090	MACF1 (microtubule-actin crosslinking factor 1)	0.098
XM_042551	CAMK2A (calcium/calmodulin-dependent protein kinase)	0.0645
NM_000909	NPY1R (neuropeptide Y receptor Y1)	0.02521
NM_015230	CENTD1 (centaurin, delta 1)	0.024
NM_004958	FRAP1 (FK506 binding protein 12-rapamycin associated protein 1)	0.0205

¹Up-regulated genes were confirmed by semiquantitative RT-PCR in another group of 15 cases;

²Down-regulated genes and aRNA from primary carcinoma tissues and their corresponding metastatic lymph nodes were labeled with Cy3-dCTP and Cy5-dCTP, respectively.