Immunohistochemical study on distribution of endocrine cells in gastrointestinal tract of flower fish (Pseudophoxinus antalyae)

Table 1 List of primary antisera used in the present study

Antiserum	Code	Dilution	Source
CGRP	sc-28920	1:200	Santa Cruz Biotech Inc.
Substance P	sc-9758	1:200	Santa Cruz Biotech Inc.
VIP	NCL-VIPp	1:200	Nova Castra Lab, UK
Bombesin	NCL-BOMp	1:200	Nova Castra Lab, UK
Somatostatin	14CL-SOMATOp	1:200	Nova Castra Lab, UK
Secretin	sc-20938	1:200	Santa Cruz Biotech Inc.
Neurotensin	sc-20806	1:200	Santa Cruz Biotech Inc.
NPY	sc-28943	1:200	Santa Cruz Biotech Inc.
TrkA	sc-118	1:200	Santa Cruz Biotech Inc.
TrkB	sc-12	1:200	Santa Cruz Biotech Inc.
TrkC	sc-117	1:200	Santa Cruz Biotech Inc.

All antisera were raised in rabbit. CGRP: Calcitonin gene related peptide; VIP: Vasoactive intestinal peptide; NPY: Neuropeptide Y.

Table 2 Mean number of endocrine cells per intestinal fold (mean ± SD) in flower fish (Pseudophoxinus antalyae) (200 intestinal folds were examined for each antisera)

Region	Calcitonin gene related peptide	Substance-P	Vasoactive intestinal polypeptide	Bombesin	Somatostatin-14	Secretin	TrkA	TrkB	TrkC	Neuropeptide Y	Neurotensin	Anova(F)	P
The enlarged area after oesophagus	5.00 ± 1.550	4.75 ± 2.217	20.50 ± 9.883	3.75 ± 2.500	8.50 ± 9.037	3.50 ± 1.291	4.00 ± 4.359	3.00 ± 100	5.67 ± 3.215	0.67 ± 0.577	4.33 ± 3.215	4.456	0.001
Anterior intestine	5.25 ± 2.500	2.75 ± 0.957	12.00 ± 6.481	2.25 ± 1.258	2.75 ± 1.500	4.25 ± 2.062	0.00 ± 0.000	2.67 ± 2.887	1.00 ± 1.000	0.00 ± 0.000	1.33 ± 0.577	6.186	0.000
Middle intestine	5.25 ± 2.217	4.25 ± 0.957	12.25 ± 6.702	1.00 ± 0.816	7.75 ± 1.708	4.75 ± 1.708	0.00 ± 0.000	1.67 ± 1.528	1.00 ± 1.000	0.67 ± 0.577	1.00 ± 1.000	8.115	0.000
Posterior intestine	4.50 ± 2.380	6.75 ± 2.217	7.25 ± 2.217	3.00 ± 0.816	0.00 ± 0.000	2.00 ± 1.44	0.00 ± 0.000	0.67 ± 1.155	0.67 ± 1.155	0.00 ± 0.000	0.33 ± 0.577	12.829	0.000