Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells

Table 1 The PCR primers and length of the amplified products

For detection	Gene	Primer sequence	Products [bp]	GenBank
			Annealing temp.[°C]	accession No.
Cynomolgus	OCT3/4	Sense: 5’-ACCACAGTCCATGCCATCAC-3’	660	Z11898
		Antisense: 5’-TCCACCACCCTGTTGCTGTA-3’	60
	Albumin	Sense: 5’-GCATCCTGATTACTCTGACATG-3’	229	AB158629
		Antisense: 5’-CTTGGTGTAACGAACTAATTGC-3’	60
	AFP	Sense: 5’-TGCAGCCAAAGTGAAGAGGGAAGA-3’	217	NM_001134
		Antisense: 5’-CATAGCGAGCAGCCCAAAGAAGAA-3’	60
	HNF4α	Sense: 5’-CCGGATCAGCACTCGAA-3’	411	NM_178849
		Antisense: 5’-AGCTCGTCAAGGATGCGTATG-3’	60
	CYP7A1	Sense: 5’-ATTTGGTGCCAATCCTCTTG-3’	312	NM_000780
		Antisense: 5’-CGTTGGAGGTTTTCCATCAT-3’	60
Cynomolgus, mouse	GAPDH	Sense: 5’-ACCACAGTCCATGCCATCAC-3’	452	NM_002046
		Antisense: 5’-TCCACCACCCTGTTGCTGTA-3’	60
mouse	OCT3/4	Sense: 5’-CGCCCGCATACGAGTTCTG-3’	678	X52437
		Antisense: 5’-GGTGTCCCTGTAGCCTCAT-3’	60
	AFP	Sense: 5’-CTTTGGACCCTCTTCTGTGA-3’	909	NM_007423
		Antisense: 5’-CACTGCTGCAACTCTTCGTA-3’	55
	Albumin	Sense: 5’-TGAACTGGCTGACTGCTGTG-3’	718	AJ457860
		Antisense: 5’-CATCCTTGGCCTCAGCATAG-3’	60
	α1AT	Sense: 5’-TGGGGTCTACTGCTTCTGG-3’	693	M25529
		Antisense: 5’-TCATGGGCACCTTCACCGT-3’	60
	HNF4α	Sense: 5’-CTAAGCTGTCCCCACAAGGCTATGCA-3’	864	NM_008261
		Antisense 5’-CAGAGCTCCACCTGGAAAGGTGTTTG-3’	60
	TDO	Sense: 5’-AGAGCCAGCAAAGGAGGAC-3’	500	BC018390
		Antisense: 5’-CTGTCTGCTCCTGCTCTGAT-3’	60
	PEPCK	Sense: 5’-TCTGCCAAGGTCATCCAGG-3’	290	AF009605
		Antisense: 5’-GTTTTGGGGATGGGCACTG-3’	60
	CYP7A1	Sense: 5’-AGGACTTCACTCTACACC-3’	453	AK050260
		Antisense: 5’-GCAGTCGTTACATCATCC-3’	56
	Desmin	Sense: 5’-ATGACCGCTTCGCCAACTA-3’	461	NM_010043
		Antisense: 5’-CATACTGAGCCCGGATGTC-3’	60
	Vimentin	Sense: 5’-TCAAGAACACCCGCACCAACGA-3’	463	NM_011701
		Antisense: 5’-GTTTGACACCTGCTTGGCCTGG-3’	60

AFP: Alpha-fetoprtein; HNF4α: Hepatic nuclear factor 4α; CYP7a1: Cytochrome P450 7A1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; α1AT: Alpha-1 antitrypsin; TDO: Tryptophan 2, 3-dioxygenase; PEPCK: phosphoenolpyruvate carboxykinase.