Expression and prognostic role of molecular markers in 99 KIT-positive gastric stromal tumors in Taiwanese

Table 1 Sources of antibodies and ratio of dilutions used in this study

Antibody	Source	Dilution
CD117 monoclonal	Dako M7140 Clone104D2	1:50
CD 34 monoclonal	Dako M7161 CloneQBEnd10	1:200
Desmin monoclonal	Novocastra NCL-DES-DERII	1:100
	Clone DE-R-11
Smooth muscle
actin monoclonal	Novocastra NCL-SMA Clone αsm	1:50
S-100 polyclonal	Novocastra NCL-S100p	1:1 500
P53 monoclonal	Dako	1:200
Ki67 monoclonal	Dako MIB1	1:50
PCNA monoclonal	Dako M0879	1:50

Table 2 The correlation between labeling index of molecular markers and pathologic parameters

	Tumor mitosis	Tumor size	P53	Ki67	PCNA
Tumor mitosis		CC = 0.319	CC = 0.488	CC = 0.394	CC = 0.657
		P = 0.0011	P < 0.0011	P < 0.0011	P < 0.0011
Tumor size	CC = 0.319		CC = 0.285	CC = 0.032	CC = 0.206
	P = 0.0011		P = 0.0041	P = 0.751	P = 0.0411
P53	CC = 0.488	CC = 0.285		CC = 0.434	CC = 0.508
	P < 0.0011	P = 0.0041		P < 0.0011	P < 0.0011
Ki67	CC = 0.394	CC = 0.032	CC = 0.434		CC = 0.295
	P < 0.0011	P = 0.751	P < 0.0011		P = 0.0031
PCNA	CC = 0.657	CC = 0.206	CC = 0.508	CC = 0.295
	P < 0.0011	P = 0.0411	P < 0.0011	P = 0.0031

CC: correlation coefficient.

¹Correlation is significant at the 0.05 level (two-tailed).

Table 3 Univariate and multivariate analyses for disease free survival rates of individual parameters of GIST patients

	Univariate			Multivariate
	Risk	95%CI	P	Risk	95%CI	P
Age ≥60/<60 yr	1.01	0.45–2.25	0.98	–	–	–
Gender M/F	2.38	1.05–5.36	0.036a	–	–	0.1
Tumor size ≥5/<5 cm	6.16	1.83–20.6	0.003a	3.43	1.12–11.8	0.025a
Mitoses ≥5/<5/50 HPF	6.30	2.49–15.9	<0.001a	3.28	1.25–8.59	0.009a
Tumor location fundic-cardiac/body-antrum	0.99	0.54–1.81	0.98	–	–	–
Hemoglobin ≥100/<100	2.21	0.86–5.63	0.095	–	–	–
Cell type spindle/non-spindle	1.23	0.73–2.08	0.42	–	–	–
Cellularity low+intermediate/high	1.26	0.82–2.16	0.38	–	–	–
Pleomorphism +/-	0.82	0.36–1.89	0.65	–	–	–
P53 +/-	12.1	2.83–51.3	0.001a	6.45	1.45–28.7	0.002a
Ki67 +/-	2.52	1.12–5.68	0.026a	–	–	0.41
PCNA above or below median	2.97	1.23–7.17	0.015a	–	–	0.42
CD 34 +/-	3.07	0.41–22.7	0.27	–	–	–
SMA +/-	1.34	0.53–3.37	0.54	–	–	–
Desmin +/-	0.74	0.17–3.16	0.68	–	–	–
S-100 +/-	2.19	0.65–1.37	0.20	–	–	–

^aP<0.05 vs others.

Table 4 Univariate and multivariate analyses for overall survival rates of individual parameters of GIST patients

	Univariate			Multivariate
	Risk	95%CI	P	Risk	95%CI	P
Age ≥60/<60 yr	0.90	0.39–2.06	0.81	–	–	–
Gender M/F	2.68	1.16–6.20	0.021a	–	–	0.097
Tumor size ≥5/<5 cm	19.01	2.56–141.1	0.004a	10.17	1.34–76.9	0.001a
Mitoses ≥5/<5/50 HPF	10.30	3.48–30.4	<0.001a	5.22	1.70–15.9	0.001a
Tumor location fundic-cardiac/body-antrum	1.07	0.58–1.96	0.81	–	–	–
Hemoglobin ≥100/<100	1.98	0.78–5.05	0.14	–	–	–
Cell type spindle/non-spindle	1.29	0.76–2.18	0.34	–	–	–
Cellularity low+intermediate/high	1.21	0.64–2.17	0.35	–	–	–
Pleomorphism +/-	0.90	0.39–2.09	0.82	–	–	–
P53 +/-	11.29	2.64–48.2	0.001a	4.84	1.08–21.6	0.012a
Ki67 +/-	2.30	1.01–5.26	0.048a	–	–	0.70
PCNA above or below median	2.75	1.13–6.70	0.025a	–	–	0.74
CD 34 +/-	1.98	0.58–6.70	0.27	–	–	–
SMA +/-	1.38	0.54–3.52	0.48	–	–	–
Desmin +/-	0.76	0.18–3.27	0.72	–	–	–
S-100 +/-	2.28	0.67–7.70	0.18	–	–	–