Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking

Table 1 Plasma 8-isoprostane in three groups with different drinking habits (means ± SD)

	Plasma 8-isoprostane (ng/L)			Group III vsGroup I	Group III vsGroup I
	Group I	Group II	Group III
Total	20.3 ± 6.1 (n = 64)	20.9 ± 5.7 (n = 56)	26.6 ± 9.5 (n = 37)	t = 4.059 P < 0.0001 (CI: 3.222-9.387)	t = 3.587 P < 0.001 (CI: 2.523-8.783)
Male	22.5 ± 6.3 (n = 32)	21.1 ± 4.9 (n = 31)	23.7 ± 8.0 (n = 20)	t = 0.589 N.S. (CI: -2.832-5.181)	t = 1.464 N.S. (CI: -0.981-6.252)
Female	18.1 ± 5.0 (n = 32)	20.8 ± 6.6 (n = 25)	30.0 ± 10.3 (n = 17)	t = 5.494 P < 0.0001 (CI: 7.572-16.321)	t = 3.542 P < 0.005 (CI: 3.954-14.466)

CI: 95% Confidence Interval.

Table 2 Spearman correlation coefficients between biochemical parameters (n = 157)

	TC	TG	HDL-C	LDL-C	apoA I	apoB	Lp(a)	AST	ALT	γ-GTP	8-isoprostane
Age	0.373	0.122	0.012	0.324	0.088	0.381	0.055	0.116	0.139	0.436	-0.062
TC	-	0.356	0.272	0.818	0.233	0.799	0.211	0.091	0.123	0.273	-0.042
TG	-	-	-0.215	0.446	-0.037	0.565	0.128	0.182	0.247	0.325	-0.020
HDL-C	-	-	-	-0.209	0.849	-0.242	-0.001	-0.068	-0.250	-0.059	-0.101
LDL-C	-	-	-	-	-0.169	0.965	0.264	0.122	0.248	0.235	0.080
apoA I	-	-	-	-	-	-0.155	-0.038	0.022	-0.121	0.083	-0.118
apoB	-	-	-	-	-	-	0.295	0.171	0.296	0.335	0.069
Lp(a)	-	-	-	-	-	-	-	0.057	0.060	-0.029	0.124
AST	-	-	-	-	-	-	-	-	0.897	0.411	-0.034
ALT	-	-	-	-	-	-	-	-	-	0.509	0.002
γ-GTP	-	-	-	-	-	-	-	-	-	-	-0.085

Table 3 Plasma 8-isoprostane analyzed by ALDH2 genotype in three groups with different drinking habits (means ± SD)

		Plasma 8-isoprostane (ng/L)			Group III vsGroup I	Group III vsGroup I
		Group I	Group II	Group III
ALDH2*1/1	Male	20.2 ± 5.8	19.8 ± 5.0	22.8 ± 5.4	t = 1.165, N.S. (CI: -1.988-7.137)	t = 1.664, N.S. (CI: -0.684-6.758)
	Female	19.0 ± 5.8	21.6 ± 6.6	26.9 ± 7.7	t = 2.754, P < 0.05 (CI: 1.926-13.808)	t = 1.897, N.S. (CI: -0.447-10.887)
	Total	19.6 ± 5.7 (n = 22)	20.6 ± 5.8 (n = 33)	24.6 ± 6.7 (n = 27)	t = 2.776, P < 0.01 (CI: 1.375-8.616)	t = 2.471, P < 0.05 (CI: 0.757-7.219)
ALDH2*1/2	Male	23.7 ± 7.5 (n = 11)	22.6 ± 4.4 (n = 12)	28.5 ± 14.8 (n = 4)	t = 0.834, N.S. (CI: -7.517-16.976)	t = 1.281, N.S. (CI: -3.934-15.600)
	Female	16.0 ± 4.0 (n = 15)	19.9 ± 7.4 (n = 8)	41.2 ± 12.3 (n =3)	t = 6.930, P < 0.0001 (CI: 17.459-32.848)	t = 3.622, P < 0.01 (CI: 8.017-34.683)
	Total	19.3 ± 6.8 (n = 26)	21.5 ± 5.8 (n = 20)	33.9 ± 14.4 (n = 7)	t = 3.898, P < 0.001 (CI: 6.973-22.276)	t = 3.267, P < 0.005 (CI: 4.586-20.221)
ALDH2*2/2	Male	22.2 ± 5.5 (n = 6)	-	-	-	-
	Female	21.8 ± 2.0 (n = 3)	-	-	-	-
	Total	22.0 ± 4.7 (n = 9)	-	-	-	-

CI: 95% confidence interval.