Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells

Table 1 Sequence of primers used for RT-PCR and length of fragments

Primer	Sequences	Product
ALB	Upper 5' TGAGAAAACGCCAGTAAGTGAC 3' Lower 5' TGCGAAATCATCCATAACAGC 3'	265 bp
AFP	Upper 5’ GCTTGGTGGTGGATGAAACA 3’ Lower 5’ TCCTCTGTTATTTGTGGCTTTTG 3’	157 bp
Thy1	Upper 5’ CACACATACCGCTCCCGAACC 3’ Lower 5’ GCTGATGCCCTCACACTTGACC 3’	189 bp
KRT 18	Upper 5’ CCCGTCACGCCCTACAGAT 3’ Lower 5’ ACCACTTTGCCATCCACTATCC 3’	271 bp
KRT 19	Upper 5’ TCCAGATGAGCAGGTCCGAGGTTA 3’ Lower 5’ GCTGCGGTAGGTGGCAATCTCC 3’	281 bp
CYP3A4	Upper 5’ CCTTACAT TACACACCCTTTGGAAGT 3’ Lower 5’ AGCTCAATGCATGTACAGAATCCCCGGTTA 3’	382 bp
CYP2E1	Upper 5’ ACAGAGACCACCAGCACAACT 3’ Lower 5’ ATGAGCGGGGAATGACACAGA 3’	580 bp
C/EBPβ	Upper 5’ CTCGCAGGTCAAGAGCAAG 3’ Lower 5’ CTAGCAGTGGCCGGAGGCGAGC 3’	271bp
HNF4α	Upper 5’ GCCTACCTCAAAGCCATCAT 3’ Lower 5’ GACCCTCCCAGCAGCATCTC 3’	275 bp
hPBGD	Upper 5’ CGGAAGAAAACAGCCCAAAGA 3’ Lower 5’ TGAAGCCAGGAGGAAGCACAGT 3’	294 bp

Table 2 Expression of CD90 and CD105 in ADSC and BMSC after the initial culture

	ADSC				BMSC
Donor	CD90+		CD105+		CD90+		CD105+
	Cellpopulation%	Meanchannelfluorescence	Cellpopulation%	Meanchannelfluorescence	Cellpopulation%	Meanchannelfluorescence	Cellpopulation%	Meanchannelfluorescence
1	99.88	8623.91	93.92	211.55	98.83	8347.11	94.85	338.36
2	99.83	5467.01	99.83	1118.22	87.69	1980.05	92.20	175.20
3	99.82	6157.88	99.08	1089.24	99.80	8538.72	96.27	129.80
4	99.89	6830.06	97.79	758.79	98.17	8700.00	89.48	622.80
5	99.78	7495.60	99.70	2025.79	98.91	8562.77	95.53	602.95
6	99.81	8052.66	99.54	1510.95	76.74	1088.24	98.70	139.73
7	98.83	8878.06	99.35	1298.27	76.42	234.83	89.20	648.32
8	91.14	5123.70	73.52	203.77	98.98	6274.51	94.90	549.53
Mean	98.62	7078.61	95.34	1027.07	91.94	5465.78	93.89	400.84
SD	3.04	1418.10	9.03	624.75	10.26	3724.95	3.33	229.88