Alteration of peripheral blood lymphocyte subsets in acute pancreatitis

Table 1 Clinical characterization of studied patients with mild AP and severe AP

	Mild APn = 20	Severe APn = 15	PM-AP vs S-AP(Mann-Whitney U test)
Etiology
Biliary	9 (45.0%)	9 (60.0%)
Alcohol	10 (50.0%)	6 (40.0%)
Idiopathic	1 (5.0%)	0 (0%)
Median ranson	1 (0-2)	4 (2-8)	P < 0.01
score (range)
Balthazar score
A	6 (30.0%)	0
B	14 (70.0%)	0
C	0	2 (13.3%)
D	0	5 (33.3%)
E	0	8 (53.3%)
CRP (mg/L) median (range)
D 1	22.9 (5.0-139.0)	216.0 (5.0-466.1)	P < 0.01
D 2	50.5 (5.2-135.4)	186.2 (43.1-342.0)	P < 0.01

Table 2 Peripheral blood lymphocyte CD7+/CD19+ and CD4+/CD8+ ratio in patients with AP

		Control	Days
			1	2	3	5	10	30
CD7+/CD19+	M-AP	6.4 ± 1.9	4.9 ± 1.2a	7.0 ± 2.3	7.6 ± 2.1	7.8 ± 2.1	7.9 ± 1.9	6.5 ± 1.7
	S-AP		4.6 ± 1.2a	8.0± 2.1a	6.3 ± 1.9	6.1 ± 1.8	15.8 ± 4.7ac	8.7 ± 2.0ac
CD4+/CD8+	M-AP	1.5 ± 0.6	1.0 ± 0.4a	1.2 ± 0.5	0.7 ± 0.6a	1.6 ± 0.5	1.2 ± 0.5	1.2 ± 0.5
	S-AP		1.4 ± 0.5c	1.4 ± 0.6	1.3 ± 0.6c	1.6 ± 0.6	0.9 ± 0.6a	1.5 ± 0.5

^aP < 0.05 vs control;

^cP < 0.05 vs mild AP at the same day of observation.

Table 3 Apoptosis and necrosis rate in lymphocytes isolated from patients with AP

	Cultured with serum (% of total)			Cultured without serum(% of total)
	Apoptosis	Living	Necrosis	Apoptosis	Living	Necrosis
Control	2 ± 1	96 ± 2	1 ± 1	23 ± 2	10 ± 3	68 ± 3
M-AP	3 ± 1	97 ± 1	1 ± 1	50 ± 6a	17 ± 6	33 ± 4a
S-AP	3 ± 1	96 ± 2	2 ± 2	50 ± 6a	15 ± 4	36 ± 5a

^aP < 0.05 vs control.

Table 4 Serum concentration of cytokines produced by Th1 lymphocyte subpopulation in patients with AP

		Control	Days
			1	2	3	5	10
IL-2	6.2 ± 5.4	M-AP	20 ± 6a	18 ± 8a	18 ± 7a	12 ± 7a	8 ± 4a
(ng/L)		S-AP	21 ± 10a	24 ± 11ac	25 ± 11ac	13 ± 9a	9 ± 5a
IFN-γ	6.0 ± 5.7	M-AP	45 ± 24a	44 ± 25a	41 ± 14a	28 ± 11a	17 ± 5a
(ng/L)		S-AP	258 ± 24ac	49 ± 23a	40 ± 19a	25 ± 10a	16 ± 6a
TNF-α	1.8 ± 2.4	M-AP	2.9 ± 1.2a	2.9 ± 1.6a	1.5 ± 0.9	0.9 ± 0.7 a	1.0 ± 0.9a
(ng/L)		S-AP	3.2 ± 1.4a	3.2 ± 1.5a	1.0 ± 1.0ac	1.0 ± 0.7a	1.1 ± 0.9a

^aP < 0.05 vs control;

^cP < 0.05 vs mild AP at the same day of observation.

Table 5 Serum concentration of cytokines produced by Th2 lymphocyte subpopulation in patients with AP

		Control	Days
			1	2	3	5	10
IL-4	6.0 ± 5.8	M-AP	164 ± 57a	133 ± 60a	79 ± 34a	82 ± 43a	60 ± 33a
(ng/L)		S-AP	198 ± 74ac	175 ± 68ac	92 ± 42ac	94 ± 45ac	72 ± 35ac
IL-5	5.2 ± 4.9	M-AP	19 ± 9a	29 ± 9a	17 ± 7a	52 ± 23ac	4 ± 3
(ng/L)		S-AP	23 ± 7a	40 ± 12ac	18 ± 7a	39 ± 19ac	3 ± 3
IL-10	1.4 ± 1.6	M-AP	40 ± 27a	50 ± 33a	49 ± 27a	32 ± 19a	39 ± 23a
(ng/L)		S-AP	52 ± 36ac	62 ± 36ac	52 ± 36a	50 ± 36ac	47 ± 31a

^aP < 0.05 vs control;

^cP < 0.05 vs Mild AP at the same day of observation.