Expression of tissue factor in pancreatic adenocarcinoma is associated with activation of coagulation

Table 1 Tissue factor expression in eight human pancreatic adenocarcinoma cell lines in relation to the histological differentiation determined by northern-blot analysis (RNA level) and by indirect immunofluorescence (protein level)

	Grade	RNA	Protein
Capan-1	I	11	2
Capan-2	I-II	3	2
AsPC-1	I-II	3	3
BxPC-3	II	3	2-3
PANC-1	II-III	1	1-2
PaCa-2	II-III	1	2-3
PaCa-3	III	11	3
PaCa-4	III	1	1-2

¹Positive only in the absence of fetal calf serum.

Table 2 Overview of oligonucleotides used for amplification of TF and asTF

Type of primer	Oligonucleotide
Forward	5´-CAGGCACTACAAATACTGTGGCAG-3´
Reverse	5´-TGCAGTAGCTCCAACAGTGCTTCC-3´

TF: Tissue factor; asTF: Alternatively spliced TF.

Table 3 Immunofluorescence staining analysis of tissue factor expression in patients with pancreatic carcinoma (PCa), chronic pancreatitis (CP) and in healthy controls (Co)

	PCa (n = 19)	CP (n = 9)	Co (n = 20)
Total immunoreactivity	17 (84%)1	9/9 (100%)2	9/20 (45%)3
Grade 3	4	0	0
Grade 2	7	0	0
Grade 1	6	9 +	0
Negative	2	0	11

¹P = 0.027 vs controls;

²A few positively stained epithelial duct cells;

³Expression restricted to subendothelial cells.

Table 4 Patients with pancreatic carcinoma complicated by clinically overt thromboembolism

Case	Sex/age	Stage1	Grade	TF2	TF (pg/mL)	Location of thromboembolism
1	m/65	4	II	3	291	Splenic vein thrombosis
2	m/69	4	II	2	nd	Mesenteric artery thrombosis
3	f/61	4	II	13	391	Upper jugular vein thrombosis
4	m/62	4	II	2	nd	Pulmonary embolism

¹ Staging according to UICC;

² Results from immunohistochemistry, i.e. immunofluorescence for TF;

³ TF was focally positive; nd: not determined.