Treatment of hepatoma with liposome-encapsulated adriamycin administered into hepatic artery of rats

Table 1 Detailed solvent gradient program for analysis of EPC and Chol by HPLC

Time (min)	0-3	3-11	11-11.5	11.5-30
A%	100	0	0	100
B%	0	100	100	0

Solution A: Chloroform-isopropanol (50:50, v/v); Solution B: Chloroform-isopropanol-water (36:55:9, v/v). EPC: egg phosphatidyl choline; Chol: Cho lesterol.

Table 2 Tumor volume (V) and tumor volume ratio (TVR) after treatment (mean ± SD)

Group	V (cm3)		TVR
	Beforetreatment	Aftertreatment
NS	0.086 ± 0.049	2.521 ± 0.840	31.550 ± 7.975
FADM	0.083 ± 0.035	0.149 ± 0.072	1.883 ± 0.708b
ADM + BL	0.079 ± 0.036	0.136 ± 0.069	1.847 ± 0.661b
LADM	0.084 ± 0.049	0.105 ± 0.062	1.243 ± 0.523d

^bP < 0.01 vs NS;

^dP < 0.01 vs FADM, ADM + BL.

Table 3 Tumor necrosis degree after treatment

Grade	NS	FADM	ADM + BL	LADM
I	6	2	1	0
II	1	3	4	3
III	0	2	2	41

¹ Complete necrosis in 2 cases.

Table 4 Mean survival time and increased life span after treatment (mean ± SD)

Group	Mean survival time (d)	% ILS
NS	11.88 ± 2.80	-
FADM	20.75 ± 6.34	74.66b
ADM + BL	23.13 ± 7.75	94.70b
LADM	39.38 ± 11.15	231.48a

% ILS : Increased life span.

^aP < 0.05 vs FADM, ADM + BL;

^bP < 0.01 vs NS.