Suppression of bile acid synthesis by thyroid hormone in primary human hepatocytes

Table 1 Formation of CA and CDCA in primary human hepatocytes obtained from 3 patients

Patient	Addition of T3	CA	CDCA	Ratio
		ng/mL	Medium/24 h	CA/CDCA
A	Control	920	595	1.5
	0.1 nmol/L	400	310	1.3
	1 nmol/L	375	375	1.0
	10 nmol/L	340	390	0.9
	100 nmol/L	250	285	0.9
	1 μmol/L	245	295	0.8
B	Control	280	60	4.7
	0.1 nmol/L	135	45	3.0
	1 nmol/L	95	70	1.4
	10 nmol/L	55	60	0.9
	100 nmol/L	45	65	0.7
	1 μmol/L	45	70	0.6
C	Control	805	502	1.6
	0.1 nmol/L	430	345	1.2
	1 nmol/L	477	392	1.2
	10 nmol/L	395	425	0.9
	100 nmol/L	372	405	0.9
	1 μmol/L	382	420	0.9

The cells were cultured in a serum-free medium and treated with different concentrations of T₃ for 5 d. Bile acids were measured in the cell-free medium. CA: Cholic acid; CDCA: Chenodeoxycholic acid.