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Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 21, 2006; 12(27): 4401-4405
Published online Jul 21, 2006. doi: 10.3748/wjg.v12.i27.4401
Table 1 OD value of pEGFP-C1-hALR detected by ultraviolet spectrophotometer
Sampleabsabsbkgabs260.0 nm280.0 nmProteinNucleic acid
ID280.0 nm260.60320.0 nm280.0 nm260.0 nmμg/mLμg/mL
10.03190.02050.00571.76790.56573.15391.1124
0.03370.02340.00861.69530.58993.96321.0440
20.01520.0073-0.00091.94860.51320.63260.7177
0.01590.0101-0.00091.84850.54101.68530.8892
Table 2 Influence of different concentrations of hALR on DNA synthesis of primary rat hepatocyte CPM (mean ± SD)
CPM1234
Without hALRcontrol group5 ng/mL hALRmedium group10 ng/mL hALRmedium group20 ng/mL hALRmedium group
n = 6n = 6n = 6n = 6
1 1125 ± 158P < 0.01P < 0.01P < 0.01
2 1802 ± 127P < 0.01P < 0.01
3 2764 ± 445P < 0.01
4 3344 ± 417
Table 3 Influence of differentconcentrations of hALR on DNA synthesis of HepG2 cell CPM ( mean ± SD)
CPM1234
Without hALRcontrol group5 ng/mL hALRmedium group10 ng/mL hALRmedium group20 ng/mL hALRmedium group
n = 6n = 6n = 6n = 6
1 1226 ± 158P < 0.01P < 0.01P < 0.01
2 1802 ± 127P < 0.01P < 0.01
3 2764 ± 445P < 0.01
4 3344 ± 417