Prognostic significance of microsatellite alterations at 1p36 in cholangiocarcinoma

Table 1 Sequences of microsatellite markers located on the chromosomal region 1p36-pter used for the LOH and MSI analysis

Marker	Sequences (5’-3’)
D1S468	F: AAT TAA CCG TTT TGG TCC T
	R: GCG ACA CAC ACT TCC C
D1S2845	F: CCA AAG GGT GCT TCT C
	R: GTG GCA TTC CAA CCT C
D1S450	F: GCT CCA ATG TCC AAG GG
	R: GGG TAC TCA GAT GGC TGG T
D1S228	F: AAC TGC AAC ATT GAA ATG GC
	R: GGG ACC ATA GTT CTT GGT GA
D1S507	F: AGG GGA TCT TGG CAC TTG G
	R: CTC TAG GGT TTC TGG AAA ATG CTG
D1S199	F: GGT GAC AGA GTG AGA CCC TG
	R: CAA AGA CCA TGT GCT CCG TA
D1S2734	F: GGT TCA AGG GAT TCT CCT G
	R: TGG CAC TCA GAC CTC AA
D1S234	F: GCC CAG GAG GTT GAG G
	R: AAG GCA GGC TTG AAT TAC AG
D1S2781	F: CTC TCA CAG ACA CAC GCA
	R: GTT CAA TGG GGG ATT CAG
D1S2676	F: TCT GTC AGA ACA AAC GTG TC
	R: GAG TTG CCA TAC TTT GCT GTA G

Table 2 Clinical features of patients with and without LOH at loci D1S2845, D1S234, and D1S2676

Clinical parameters	D1S2845				D1S234				D1S2676
	n1	WithoutLOH n (%)	WithLOH n (%)	P	n1	WithoutLOH n (%)	WithLOH n (%)	P	n1	WithoutLOH n (%)	WithLOH n (%)	P
Number of patients	82	57 (69.5)	25 (30.5)		80	62 (77.5)	18 (22.5)		80	64 (80.0)	16 (20.0)
Age range (yr)
32-53	36	23 (63.9)	13 (36.1)	NS	34	26 (76.5)	8 (23.5)	NS	37	31 (83.8)	6 (16.2)	NS
54-75	46	34 (73.9)	12 (26.1)		46	36 (78.3)	10 (21.7)		43	33 (76.7)	10 (23.3)
Sex
Male	55	38 (69.1)	17 (30.9)	NS	54	42 (77.8)	12 (22.2)	NS	54	41 (75.9)	13 (24.1)	NS
Female	27	19 (70.4)	8 (29.6)		26	20 (76.9)	6 (23.1)		26	23 (88.5)	3 (11.5)
Histological type
Papillary	19	12 (63.2)	7 (36.8)	NS	17	10 (58.8)	7 (41.2)	0.035	20	17 (85.0)	3 (15.0)	NS
Well diff	22	15 (68.2)	7 (31.8)		23	20 (87.0)	3 (13.0)		22	17 (77.3)	5 (22.7)
Moderately diff	11	7 (63.6)	4 (36.4)		11	10 (90.9)	1 (9.1)		12	11 (91.7)	1 (8.3)
Poorly diff	18	14 (77.8)	4 (22.2)		18	16 (88.9)	2 (11.1)		15	11 (73.3)	4 (26.7)
Rare phenotype2	12	9 (75.0)	3 (25.0)		11	6 (54.5)	5 (45.5)		11	8 (72.7)	3 (27.3)
Blood vessel invasion
Invasion	52	40 (76.9)	12 (23.1)	NS	50	39 (78.0)	11 (22.0)	NS	50	40 (80.0)	10 (20.0)	NS
Non-invasion	30	17 (56.7)	13 (43.3)		30	23 (76.7)	7 (23.3)		30	24 (80.0)	6 (20.0)
Nerve invasion
Invasion	36	20 (55.6)	16 (44.4)	0.029	36	30 (83.3)	6 (16.7)	NS	37	27 (73.0)	10 (27.0)	NS
Non-invasion	46	37 (80.4)	9 (19.6)		44	32 (72.7)	12 (27.3)		43	37 (86.0)	6 (14.0)
Lymphatic invasion
Invasion	58	38 (65.5)	20 (34.5)	NS	56	48 (85.7)	8 (14.3)	0.017	57	42 (73.7)	15 (26.3)	0.031
Non-invasion	24	19 (79.2)	5 (20.8)		24	14 (58.3)	10 (41.7)		23	22 (95.7)	1 (4.3)
Survival mean (wk)	82	57.53	34.97	NS	80	59.72	39.23	NS	80	59.78	28.52	NS

¹Number of informative cases;

²Squamous and adenosquamous carcinoma.