Antioxidative effect of melatonin, ascorbic acid and N-acetylcysteine on caerulein-induced pancreatitis and associated liver injury in rats

Table 1 Scores for pancreatic and hepatic damage in different groups (mean ± SE)

Group	Scores forpancreatic damage	Scores forhepatic damage
Control	0.00 ± 0.00	0.00 ± 0.00
Caerulein	6.16 ± 0.67a	6.28 ± 0.56a
Caerulein + melatonin	2.14 ± 0.70c	1.14 ± 0.26c
Caerulein + antioxidant mixture	1.85 ± 0.45c	1.42 ± 0.36c

^aP < 0.005 vs control; ^cP < 0.005 vs caerulein.

Table 2 MDA, CAT and after treatment GPx levels in pancreatic tissues of different groups after treatment (mean ± SE)

Groups	MDA(nmol/mg pr)	CAT(U/mg pr)	GPx(U/mg pr)
Control	1.74 ± 0.23	10.29 ± 0.68	0.59 ± 0.05
Caerulein	2.63 ± 0.22a	2.78 ± 0.28g	0.10 ± 0.00g
Caerulein + melatonin	1.90 ± 0.17c	15.63 ± 0.67e	1.72 ± 0.29e
Caerulein + antioxidant mixture	1.30 ± 0.15e	7.02 ± 1.84	1.15 ± 0.18

^aP < 0.05 vs control; ^cP < 0.05 vs caerulein; ^eP < 0.005 vs caerulein; ^gP < 0.005 vs control.

Table 3 MDA, CAT and GPx levels in liver tissues of different groups after treatment (mean ± SE)

Group	MDA(nmol/mg pr)	CAT(U/mg pr)	GPx(U/mg pr)
Control	7.00 ± 2.29	3.20 ± 0.83	0.72 ± 0.25
Caerulein	20.89 ± 10.13a	1.09 ± 0.35a	0.33 ± 0.09g
	9.66 ± 3.65c	3.69 ± 1.35e	0.50 ± 0.12c
Caerulein + antioxidant mixture	10.49 ± 3.03c	3.12 ± 1.02e	0.61 ± 0.26c

^aP < 0.005 vs control; ^cP < 0.05 vs caerulein; ^eP < 0.005 vs caerulein, ^gP < 0.05 vs control.

Table 4 AST, ALT, LDH, total and direct bilirubin levels in different groups after treatment (mean ± SE)

Group	AST(U/L)	ALT(U/L)	LDH(U/L)	Total bilirubin(mg/dL)	Direct bilirubin(mg/dL)
Control	186.85 ± 19.5	53.71 ± 2.39	4088.43 ± 510.5	0.02 ± 0.02	0.01 ± 0.01
Caerulein	259.57 ± 18.60a	68.14 ± 4.04a	7631.14 ± 281.3c	6.88 ± 0.12c	3.06 ± 0.09c
Caerulein + mel	193.28 ± 26.64	47.71 ± 6.98	5065.71 ± 508.10	0.04 ± 0.01g	0.00 ± 0.01g
Caerulein + antioxidant mixture	274.71 ± 17.16	70.14 ± 4.84e	6592.71 ± 1065.41	0.05 ± 0.02g	0.04 ± 0.02g

^aP < 0.05 vs control; ^cP < 0.005 vs control; ^eP < 0.05 vs caerulein+mel; ^gP < 0.005 vs caerulein.

Table 5 Serum amylase and lipase levels in different groups after treatment (mean ± SE)

Group	Amylase(U/L)	Lipase(U/L)
Control	379.28 ± 15.71	22.71 ± 0.68
Caerulein	2182.29 ± 563.92a	117.0 ± 7.56c
Caerulein + mel	1406.71 ± 169.44	65.0 ± 10.81
Caerulein + antioxidant mixture	1881.57 ± 414.20	70.71 ± 8.72

^aP < 0.05 vs control; ^cP < 0.005 vs control