Antibody to E1 peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro

Table 1 Neutralizing activity of E1 antibody on infectivity of HCV to HepG2 cells

n	Viral Load	Genotype	Pre-incubation
			PBS	Anti E1 Ab
1	< 615	4c/4d	positive	positive
2	1 481 570	4	positive	positive
3	28 469	4c/4d	Cells not infected1	Cells not infected1
4	4 030	4e	Cells not infected1	Cells not infected1
5	42 154	4	positive	positive
6	114 598	4	positive	negative
7	< 615	4c/4d	positive	positive
8	-	1b	Cells not infected1	Cells not infected1
9	2 835 590	4a	positive	negative
10	110 933	4c/4d	positive	negative
11	1 052 310	4c/4d	positive	negative
12	806 482	4e	positive	negative
13	4 721	4c/4d	positive	negative
14	817 331	4	Cells not infected1	Cells not infected1
15	2 077	4a	Positive	negative
16	177 021	4	Positive	negative
17	147 226	4c/4d	Cells not infected1	Cells not infected1
18	3 284 580	4c/4d	Positive	negative
19	1 136 230	4	Positive	negative
20	506 773	4c/4d	Cells not infected1	Cells not infected1
21	380 695	4	Cells not infected1	Cells not infected1
22	159725	4	Positive	negative
23	1216640	4e	Positive	negative
24	< 615	4a	Cells not infected1	Cells not infected1
25	< 615	4c/4d	Positive	positive
26	284 962	4	Cells not infected1	Cells not infected1
27	336 705	4a	Positive	negative
28	< 615	4	Cells not infected1	Cells not infected1

The presence of HCV RNA was determined by nested RT-PCR and genotyped using Innolipa system (Bayer, Germany). Viral loads were determined by branched DNA method (Bayer, Germany). PBS: phosphate buffer saline

¹Cells not infected: HepG2 cells did not infected by positive HCV serum.